Effects of Atractylodes macrocephala on the cytomembrane Ca²⁺-activated K⁺ currents in cells of human pregnant myometrial smooth muscles

Abstract

The study examined the inhibitory effect of Atractylodes macrocephala (AM) on the uterine contraction during premature delivery and explored its electrophysiological mechanism by studying the effects of AM on the Ca²⁺-activated K⁺ currents of pregnant human myometrial smooth muscle cells with or without the treatment with interleukin-6. Single cells were acutely isolated from pregnant human myometrial smooth muscles. Whole-cell Ca²⁺-activated K⁺ currents were recorded by using an Axopatch1-D amplifier. The cells were divided into three groups: group A in which AM was added into perfusate, group B, in which interleukin-6 was added into perfusate) and group C in which AM was added into perfusate after addition of interleukin-6. IL-6 10 ng/mL inhibited BK_ca by 36.9%±13.7% as compared with control (P<0.01). AM at 2 mg/mL raised BK_ca by 36.7%±22.6% or 45.2%±13.7% with or without the treatment of IL-6, respectively (P<0.01). It is concluded that AM was able to enhance the BK_ca of pregnant human myometrial smooth muscle cells treated or untreated with interleukin-6 and its effect on the BK_ca IL-treated cells was stronger that its effect on BK_ca of untreated cells. Our results suggested that AM can help to maintain the membrane potentials and the resting status of pregnant human myometrial smooth muscle cells.

Keywords

atractylodes macrocephala / interleukin-6 / patch clamp / Ca²⁺-activated K⁺ currents / myometrial smooth muscle cells / premature delivery