Comparative study on the effects of self-made liver preservation solution on secretion of ICAM-1 and NO in rats

Abstract

In order to study the effect of self-made liver preservation solution on liver preservation by comparing with UW solution and HC-A solution, the self-made liver preservation solution (SM) and perfusion solution were prepared under the aseptic conditions. The isolated non-circulated perfusion rat liver model was established. According to the different preservation solutions, the rats were randomly divided into UW group, SM group and HC-A group. The three groups were divided into 6 subgroups according to the preservation duration (n=6 in each group). The transferase in liver perfusion solution and intercellular adhesion molecule-1 (ICAM-1) and nitric oxide (NO) in liver tissues were determined at 2, 8 and 24 h respectively. The results showed that the levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) had no significant difference between SM group and UW group, but significantly lower than in HC-A group. The levels of ICAM-1 and NO were increased simultaneously in SM group and UW group (P>0.05), but there was significant difference as compared with HC-A group (P<0.05). At the same time point, the level of ICAM-1 was higher in SM group than in UW group, but NO was lower. The preservation effect of SM solution is the same as UW solution, but better than HC-A solution.

Keywords

preservation solution / liver transplantation / intercellular adhesion molecule-1 / nitric oxide

References

[1] NishimuraY., TakeiY., KawanoS., et al.. The F (ab’) 2 fragment of an anti-ICAM monoclonal antibody attenuates liver injury after orthotopic liver transplantation. Transplantation, 1996, 61: 99-104

[2] TakeiY., MarziI., KauffmanF. C., et al.. Leukocyte adhesion and cell death following orthotopic liver transplantation in the rat. Transplantation, 1991, 59: 959

[3] ZeiniM., hortelanoS., TravesP. G., et al.. Assessment of a dual regulatory role for NO in liver regeneration after partial hepatectomy: protection against apoptosis and retardation of hepatocyte proliferation. FASEB J, 2005, 19(8): 995-997

[4] OikawaK., OhkohchiN., SatoM., et al.. Kupffer cells play an important role in the cytokine production and activation of nuclear factors of liver grafts from non-heart-beating donors. Transplant Int, 2002, 15(8): 397-405