Establishment of mus skin photo-damage model by 8-MOP plus UVA irradiation

Abstract

To establish a simple and reliable animal model of skin photo-damage, 20 mice were treated with 8-MOP and exposed to UVA (UVA 320–400 nm) for 24 h. After irradiation, the structure of the epidermis and dermis, collagen fibers, elastic fibers were observed by using HE staining and Weigert technique and compared with the normal controls. The acanthosis and epidemis proliferation with accompanying hyperkeratosis and parakeratosis were observed. Inflammatory infiltration was noted in the dermis. The elastic fibers became coarse, irregularly arranged and clustered, with their number increased. The collagen fibers showed obvious degeneration and some amorphous materials could also be observed. The blood vessels were irregularly dilated and vascular walls were thickened, with infiltration of inflammatory cells. It is concluded that murine photodamage model can be quickly, conveniently and reliably established by means of 8-MOP/UVA.

Keywords

skin photodamage / 8-MOP/UVA / animal model

References

[1] Liu W. Photo-age of skin. Clin Dermatol J (Chinese), 200,32(7):1–2

[2] JinY. S., EunK. K., SooH. L., et al.. Enhanced expression of cylooxygenase-2 by UV in aged human skin. Mech Aging Dev, 2003, 124: 903-910

[3] JiH. H., MinS. R., ParkC. H., et al.. Selective COX-2 inhibitor, NS398, inhibits the replicative senescence of cultured dermal broblasts. Mech Aging Dev, 2004, 125: 359-366

[4] LiuZ. R., LiuR. Q., ZhangG. W., et al.. Study on the DNA mutation of skin fibroblasts induced by 8-MOP and long-wavelength ultraviolet light. Chin J Gerontol (Chinese), 2002, 21(6): 408-418