Effective penetration of cell-permeable peptide mimic of tyrosine residue 654 domain of β-catenin into human renal tubular epithelial cells
Rui Zeng , Gang Xu , Min Han , Wei Liu , Xiaocheng Liu
Current Medical Science ›› 2007, Vol. 27 ›› Issue (2) : 630 -634.
Effective penetration of cell-permeable peptide mimic of tyrosine residue 654 domain of β-catenin into human renal tubular epithelial cells
Phosphorylation of β-catenin tyrosine residue 654 plays an important role in the epithelial to myofibroblast transition (EMT). Introducing mimic peptide of tyrosine residue 654 domain of β-catenin into cells may influence phosphorylation of β-catenin tyrosine residue 654. To deliver this mimic peptide into renal epithelial cells, we used penetratin as a vector, which is a novel cell permeable peptide, to deliver hydrophilic molecules into cells. A tyrosine 654 residue domain mimic peptide of β-catenin (PM) with fused penetratin was constructed, purified and then detected for the penetration of the mimic peptide into human renal tubular epithelial cells (HK-2). The results showed that purified fusion mimic peptide could efficiently and rapidly translocate into human renal tubular epithelial cells. It is concluded that a cell-permeable peptides mimic of tyrosine residue 654 domain of β-catenin was successfully obtained, which may provide a useful reagent for interfering the human renal tubular epithelial-mesenchymal transition.
β-catenin / mimic peptide / cell permeable peptide / expression / purification / penetration
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| [6] |
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| [7] |
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| [8] |
|
| [9] |
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| [10] |
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| [11] |
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| [12] |
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| [13] |
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| [14] |
|
| [15] |
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| [16] |
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| [17] |
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| [18] |
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| [19] |
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| [20] |
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| [21] |
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| [22] |
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| [23] |
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| [24] |
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| [25] |
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| [26] |
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| [27] |
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