The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells

Nengxing Lin; Changzheng Huang; Jin Tian; Juan Tao; Jin Zhang; Lingyun Yang; Yan Li; Yeqiang Liu; Siyuan Chen; Guanxin Shen; Jiawen Li; Chunsen Wang; Yating Tu

doi:10.1007/s11596-007-0535-x

Current Medical Science ›› 2007, Vol. 27 ›› Issue (35) : 611 -613. DOI: 10.1007/s11596-007-0535-x

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The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells

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Abstract

In order to investigate the expression of endothelin receptor B (ETR-B) in human malignant melanoma (MM) cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 (ET3) on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.

Keywords

melanoma / endothelin receptor B / endothelin 3 / A375 / SK-mel-1

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Nengxing Lin, Changzheng Huang, Jin Tian, Juan Tao, Jin Zhang, Lingyun Yang, Yan Li, Yeqiang Liu, Siyuan Chen, Guanxin Shen, Jiawen Li, Chunsen Wang, Yating Tu. The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells. Current Medical Science, 2007, 27(35): 611-613 DOI:10.1007/s11596-007-0535-x

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[1]	BaynashA. G., HosodaK., GiaidA., et al.. Interaction of endothelin-3 with endothelin-B receptor is essential for development of epidermal melanocytes and enteric neurons. Cell, 1994, 79(7): 1277-1285

[2]	DupinE., Le DouarinN. M.. Development of melanocyte precursors from the vertebrate neural crest. Oncogene, 2003, 22(20): 3016-3023

[3]	YohnJ. J., SmithC., StevensT., et al.. Human melanoma cells express functional endothelin-1 receptors. Biochem Biophys Res Commun, 1994, 201(1): 449-457

[4]	DemunterA., De Wolf-PeetersC., DegreefH., et al.. Expression of the endothelin-B receptor in pigment cell lesions of the skin. Evidence for its role as tumor progression marker in malignant melanoma. Virchows Arch, 2001, 438(5): 485-491

[5]	LahavR., HeffnerG., PattersonP. H.. An endothelin receptor B antagonist inhibits growth and induces cell death in human melanoma cells in vitro and in vivo. Proc Natl Acad Sci U S A, 1999, 96(20): 11496-11500