The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells
Nengxing Lin , Changzheng Huang , Jin Tian , Juan Tao , Jin Zhang , Lingyun Yang , Yan Li , Yeqiang Liu , Siyuan Chen , Guanxin Shen , Jiawen Li , Chunsen Wang , Yating Tu
Current Medical Science ›› 2007, Vol. 27 ›› Issue (35) : 611 -613.
The expression of endothelin receptor B in melanoma cells A375 and Sk-mel-1 and the proliferative effects of endothelin 3 on A375 cells
In order to investigate the expression of endothelin receptor B (ETR-B) in human malignant melanoma (MM) cells A375 and SK-mel-1 and the proliferative effects of endothelin 3 (ET3) on A375 cells, RT-PCR was applied to detect the expression of ETR-B gene in human MM cells A375 and SK-mel-1. MTT method was used to evaluate the growth enhancing effects of ET3 on A375 cell line in vitro. The results showed that ETR-B gene was expressed in both MM A375 and SK-mel-1 cells. ET3 had stronger ability to enhance the proliferation of A375 cells in vitro in a concentration-dependent manner. It was suggested that ET3/ETR-B might play an important proliferative role in MM.
melanoma / endothelin receptor B / endothelin 3 / A375 / SK-mel-1
/
| 〈 |
|
〉 |
PDF
