The expression levels of endogenous aFGF mRNA in microwave burn wound tissues and its clinical significance

Abstract

The expression levels and changes of endogenous acid fibroblast growth factor (aFGF) in microwave burn wound tissues were detected in order to investigate how to get better therapeutic effects by using the exogenous aFGF for repairing trauma. A burnt-wound animal model was established by NS-F multifunction spectrum therapeutics equipment, and reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry assay were applied to detect the expression levels of endogenous aFGF mRNA in microwave burn wound tissues. The expression level of endogenous aFGF mRNA was significantly increased in the burn wound tissues 12 h after burn, reached the peak at 48 h, and gradually deceased 96 h after burn. The expression of endogenous aFGF mRNA after tissue damage was reversible, and its intensity was in accordance with the repair process of tissue damage, suggesting endogenous aFGF may take part in the cell metabolism and proliferation, and then promote the repair of the burn wound.

Keywords

aFGF / repair in trauma / expression of endogenous aFGF mRNA

References

[1] DipietroL. A.. Wound healing: The role of the macrophage and other growth factors. Shock, 1997, 913(3): 179-199

[2] XuL. S., LeJ. F.. Recovery of Burn Wound, 2000, Wuhan, Science and Technology Press of Hubei: 10-21

[3] WilliamsW. G., PhillipsL. G.. HerndonD. N.. Pathophysiology of the burn wound. Total burn care, 2000, London, WB Saunders Company Ltd.: 63-70

[4] MullerM. J., GilpinD. A., HerndonD. N., et al.. Modulation of wound healing and the postburn response. Total burn care, 2000, London, WB Saunders Company Ltd.: 223-236

[5] LauraR. B., AnneN. W.. Terminal complement proteins C56-9 released basic fibroblast growth factor and platelet derived growth factor from endothelial cells. Exper Med, 1994, 179: 85-88

[6] M’HoffertU., WingertszahnJ., WiedowO.. Human leukocyte elastase induces keratinocyte proliferation by epidermal growth factor receptor activation. Invest Dermatol, 2004, 123: 338-345

[7] Park J E, Barbul A. Understanding the role of immune regulation in wound healing. Am J Surg, 2004,(Suppl):11–16

[8] BairdA., MormedeP.. Molecular characterization of fibroblast growth factor: distribution and biological activities in various tissues. Recent Program Res, 1986, 42: 143-205