Expression of Candida albicans secreted aspartyl proteinase in acute vaginal candidiasis

Abstract

In order to analyze the in vivo expression of Candida albicans secreted aspartyl proteinases (SAP) in human vaginal infection, the vaginal secretion from 29 human subjects was collected by vaginal swab, and the expression of SAP1-SAP6 was detected by reverse-transcriptase polymerase chain reaction using specific primer sets. It was found that Sap2 and Sap5 were the most common genes expressed during infection; Sap3 and Sap4 were detected in all subjects and all 6 SAP genes were simultaneously expressed in some patients with vaginal candidiasis. It was suggested that the SAP family is expressed by Candida albicans during infection in human and that Candida albicans infection is associated with the differential expression of individual SAP genes which may be involved in the pathogenesis of vaginal candidasis.

Keywords

candidiasis / vulvovaginal / Candida albicans / secreted aspartyl proteinase

References

[1] HubeB., NaglikJ. R.. CalderoneR. A.. Extracellular hydrolases. Candida and candidiasis, 2002, Washington DC, American Society for Microbiology Press: 107-122

[2] HubeB., NaglikJ.. Candida albicans proteinases: resolving the mystery of a gene family. Microbiology, 2001, 147: 1997-2005

[3] HubeB.. ErnstJ. F., SchmidtA.. Extracellular proteinases of human pathogenic fungi. Contributions to microbiology: dimorphism in human pathogenic and apathogenic yeasts, 2000, Basel, S. Karger AG: 126-137

[4] NaglikJ. R., NewportG., WhiteT. C., et al.. In vivo analysis of secreted aspartyl proteinase expression in human oral candidiasis. Infect Immun, 1999, 67: 2482-2490

[5] LosbergerC., ErnstJ. F.. Sequence of the Candida albicans gene encoding actin. Nucleic Acids Res, 1989, 17: 9488

[6] CobbB. D., ClarksonJ. M.. A simple procedure for optimizing the polymerase chain reaction (PCR) using modified Taguchi methods. Nucleic Acids Res, 1994, 22: 3801-3805

[7] WhiteT. C., AgabianN.. Candida albicans secreted aspartyl proteinases: isoenzyme pattern is determined by cell type, and levels are determined by environmental factors. J Bacteriol, 1995, 177: 5215-5221

[8] ColinaA. R., AumontF., DeslauriersN., et al.. Evidence for degradation of gastrointestinal mucin by Candida albicans secretory aspartyl proteinase. Infect Immun, 1996, 64: 4514-4519

[9] RipeauJ. S., FiorilloM., AumontF., et al.. Evidence for differential expression of Candida albicans virulence genes during oral infection in intact and human immunodeficiency virus type 1-transgenic mice. J Infect Dis, 2002, 185: 1094-1102

[10] StaibP., KretschmarM., NichterleinT., et al.. Differential activation of a Candida albicans virulence gene family during infection. PNAS, 2000, 97: 6102-6107

[11] SchallerM., SchäferW., KortingH. C., et al.. Differential expression of secreted aspartyl proteinases in a model of human oral candidiasis and in patient samples from the oral cavity. Mol Microbiol, 1998, 29: 605-615

[12] De bernardisF., CassoneA., SturtevantJ., et al.. Expression of Candida albicans Sap1 and Sap2 in experimental vaginitis. Infect Immun, 1995, 63: 1887-1892

[13] SchallerM., KortingH. C., SchaferW., et al.. Secreted aspartic proteinase (Sap) activity contributes to tissue damage in a model of human oral candidiasis. Mol Microbiol, 1999, 34: 169-180