Effect of simvastatin on IL-6 and adiponectin secretion and mRNA expression in 3T3-L1 adipocytes

Abstract

In order to investigate the effects of simvastatin on secretion and mRNA expression of interleukin-6 (IL-6) and adiponectin in 3T3-L1 adipocytes, mouse 3T3-L1 adipocytes were stimulated with lipopolysaccharide (LPS). Production and mRNA expression of IL-6 and adiponectin in 3T3-L1 adipocytes were measured using enzyme-linked immunosorbent assay (ELISA) and reverse transcriptase polymerase chain reaction (RT-PCR), respectively. The results showed that simvastatin could significantly suppress LPS-induced IL-6 production and mRNA expression in adipocytes (P<0.05), but increase the LPS-induced adiponectin secretion and mRNA expression in a dose-dependent manner (P<0.05). It was suggested that simvastatin could exert beneficial effects on prevention of obesity-induced metabolic changes in adipocytes.

Keywords

obesity / adipocyte / adiponectin / simvastatin / interleukin-6

References

[1] WellenK. E., HotamisligilG. S.. Obesity-induced inflammatory changes in adipose tissue. J Clin Invest, 2003, 112: 1785-1788

[2] MatsuzawaY., FunahashiT., NakamuraT.. Molecular mechanism of metabolic syndrome X: contribution of adipocytokines adipocyte-derived bioactive substances. Ann N Y Acad Sci, 1999, 892: 146-154

[3] MaedaK., OkuboK., ShimomuraI., et al.. cDNA cloning and expression of a novel adipose specific collagen-like factor, apM1 (AdiPose Most abundant Gene transcript 1). Biochem Biophys Res Commun, 1996, 221: 286-289

[4] WoodsA., BrullD. J., HumphriesS. E., et al.. Genetics of inflammation and risk of coronary artery disease: the central role of interleukin-6. Eur Heart J, 2000, 21: 1574-1583

[5] BernlohrD. A., AngusC. W., LaneM. D., et al.. Expression of specific mRNAs during adipose differentiation: identification of an mRNA encoding a homologue of myelin P2 protein. Proc Natl Acad Sci U S A, 1984, 81: 5468-5472

[6] DasU. N.. Pathophysiology of metabolic syndrome X and its links to the perinatal period. Nutrition, 2005, 21: 762-773

[7] YudkinJ. S., StehouwerC. D., EmeisJ. J., et al.. C-reactive protein in healthy subjects: associations with obesity, insulin resistance, and endothelial dysfunction: a potential role for cytokines originating from adipose tissue?. Arterioscler Thromb Vasc Biol, 1999, 19: 972-978

[8] BulloM., Garcia-LordaP., MegiasI., et al.. Systemic inflammation, adipose tissue tumor necrosis factor, and leptin expression. Obes Res, 2003, 11: 525-531

[9] RotterV., NagaevI., SmithU.. Interleukin-6 (IL-6) induces insulin resistance in 3T3-L1 adipocytes and is, like IL-8 and tumor necrosis factor-alpha, overexpressed in human fat cells from insulin-resistant subjects. J Biol Chem, 2003, 278: 45 777-45 784

[10] FasshauerM., KleinJ., LossnerU., et al.. Interlenkin (IL)-6 mRNA expression is stimulated by insulin, isoproterenol, tumour necrosis factor alpha, growth hormone and IL-6 in 3T3-L1 adipocytes. Horm Metab Res, 2003, 35: 147-152

[11] FriedS. K., BunkinD. A., GreenbergA. S., et al.. Omental and subcutaneous adipose tissues of obese subjects release interleukin-6: depot difference and regulation by glucocorticoid. J Clin Endocrinol Metab, 1998, 83: 847-850

[12] ChenC., LiZ., LiJ.. Anti-inflammatory effect of cerivastatin in vascular injury independent of serum cholesterol and blood pressure lowering effects in mouse model. Chin J Traumatol (Chinese), 2002, 5: 294-298