Irradiation injury temporarily induces enhancement of APN/CD13 peptidase activity on aorta-gonads-mesonephros (AGM)-derived stromal cells

Yan Zhu; Lifang Huang; Xiaohua Luo; Hanying Sun; Dan Ran; Kejie Zhang; Miao Zheng; Kun Zhou; Wenli Liu

doi:10.1007/s11596-007-0209-8

Current Medical Science ›› 2007, Vol. 27 ›› Issue (9) : 145 -148. DOI: 10.1007/s11596-007-0209-8

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Irradiation injury temporarily induces enhancement of APN/CD13 peptidase activity on aorta-gonads-mesonephros (AGM)-derived stromal cells

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Abstract

This study was designed to investigate the expression of aminopeptidase N (APN)/CD13 on intraembryonic AGM stromal cells, and the change of its enzymatic activity after irradiation injury. The expression of APN/CD13 on AGM stromal cells was assayed by RT-PCR and immunihistochemistry. After the stromal cells in AGM region were irradiated with 8.0 Gy of ⁶⁰Co γ-rays, APN/CD13 enzymatic activity was measured by spectrophotometer at different time points. The result showed that AGM stromal cells strongly expressed APN/CD13. The enzymatic activity of APN/CD13 decreased temporarily after irradiation injury, then increased to higher level 4 h after irradiation, and it returned to the pre-irradiation level 24 to 48 h after the irradiation. The enzymatic activity of APN/CD13 was temporarily enhanced after irradiation injury, which might be one of the compensatory mechanisms that promote the hematopoietic recovery after irradiation.

Keywords

APN/CD13 / aorta-gonads-mesonephros (AGM) region / stromal cell / irradiation injury / enzymatic activity

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Yan Zhu,Lifang Huang,Xiaohua Luo,Hanying Sun,Dan Ran,Kejie Zhang,Miao Zheng,Kun Zhou,Wenli Liu. Irradiation injury temporarily induces enhancement of APN/CD13 peptidase activity on aorta-gonads-mesonephros (AGM)-derived stromal cells. Current Medical Science, 2007, 27(9): 145-148 DOI:10.1007/s11596-007-0209-8