Effect of interleukin-1β on I_A and I_K currents in cultured murine trigeminal ganglion neurons

Abstract

To investigate the effect of interleukin-1β (IL-1β) on I_A and I_K currents in cultured murine trigeminal ganglion (TG) neurons, whole-cell patch clamp technique was used to record the I_A and I_K currents before and after 20 ng/mL I_L-1β perfusion. Our results showed that 20 ng/mL IL-1β inhibited I_A currents (18.3 ± 10.7)% (n=6, P<0.05). I_L-1β at 20 ng/mL had no effect on G-V curve of I_A but moved the H-infinity curve V0.5 from −36.6±6.1 mV to −42.4±5.2 mV (n=5, P<0.01). However, 20 ng/mL IL-1β had effect on neither the amplitude nor the G-V curve of I_K. IL-1β was found to selectively inhibit I_A current in TG neurons and the effect may contribute to hyperalgesia under various inflammatory conditions.

Keywords

I_L-1β / trigeminal ganglion neurons / I_A current (rapidly activating, rapidly inactivating potassium current) / I_K current (delayed rectifier potassium current)

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