Relationship between the changes of VEGF level and dendritic cells in peripheral blood of patients with hepatocellular carcinoma after transcatheter arterial chemoembolization

Abstract

In order to investigate the relationship between the VEGF level and the counts of dendritic cells (DCs) in peripheral blood of patients with hepatocellular carcinoma (HCC) before and after transcatheter arterial chemoembolization (TACE), the peripheral blood was obtained from 37 patients with HCC who treated by TACE. The blood was obtained on the day before TACE, the first day, the 7th day and the 15th day after TACE respectively. The counts of DCs were quantified by flow cytometry. The plasma VEGF level was measured by ELESA kit. It was shown after TACE, the counts of DCs in peripheral blood were decreased significantly (P<0.05), and the VEGF level in peripheral blood was increased significantly (P<0.05). The counts of DCs in peripheral blood had an inverse correlation with the plasma VEGF level (r=−0.57, P<0.05) after TACE. It was concluded that in patients with HCC after TACE, the increased plasma VEGF level appeared to have the effect to suppress the maturation of DCs, which may contribute to reduction of the body’s anti-tumor immunity effect, with a consequence of recur and metastasis of tumor.

Keywords

hepatocellular carcinoma / chemoembolization / dendritic cells / VEGF

References

[1] VollstedtS., FranchiniM., HeftiH. P., et al.. Flt3 ligand treated neonatal mice have increased innate immunity against intracellular pathogens and efficiently control virus infection. J Exp Med, 2003, 197(5): 575-584

[2] IyodaT., ShimoyamaS., KangL., et al.. The CD8+ dendritic cell subset selectively endocytoses dying cells in culture and in vivo. J Exp Med, 2002, 195(10): 1289-1302

[3] YinX. Y., LuM. D., LaiY. R., et al.. Prognostic significances of tumor-infiltrating S-100 positive dendritic cells and lymphocytes in patients with hepatocellular carcinoma. Hepatogastroenterology, 2003, 50(53): 1281-1284

[4] TroyA., DavidsonP., AtkinsonC., et al.. Phenotypic characterisation of the dendritic cell infiltrate in prostate cancer. J Urol, 1998, 160(1): 214-219

[5] ZhouJ., TangZ. Y., FanJ., et al.. Expression of platelet-derived endothelial cell growth factor and vascular endothelial growth factor in hepatocellular carcinoma and portal vein tumor thrombus. J Cancer Res Clin Oncol, 2000, 126: 57-61

[6] RichardD. E., BerraE., PouyssegurJ.. Angiogenesis: how a tumor adapts to hypoxia. Biochem Biophys Res Commun, 1999, 266(37): 718-722

[7] AlmandB., ResserJ. R., LindmanB., et al.. Clinical significance of defective dendritic cell differentiation in cancer. Clin Cancer Res, 2000, 6: 1755-1766

[8] OhmJ. E., ShurinM. R., EscheC.. Effect of vascular endothelial growth factor and FLT3 ligand on dendritic cell generation in vivo. J Immunol, 1999, 163(6): 3260-3268

[9] UmS. H., MulhallC., AlisaA., et al.. Alpha-fetoprotein impairs APC function and induces their apoptosis. J Immunol, 2004, 173(3): 1772-1778