Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat

Abstract

To investigate the technique of sorting high-purity precartilaginous stem cells from rat’s perichondrium, neonatal rat’s perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purity of the sorted neural stem cells was found to be 93.0 %–99.0 %, with living cells amounting to 80 %–85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.

Keywords

magnetic beads / precartilaginous stem cell (PCSC) / anti-fibroblast growth factor receptor-3 (anti-FGFR-3)

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