Immunomagnetic indirect positive sorting of precartilaginous stem cells from neonatal rat

Abstract

To investigate the technique of sorting high-purity precartilaginous stem cells from rat’s perichondrium, neonatal rat’s perichondrium cells suspensions were incubated with monoclone antibody of anti-fibroblast growth factor receptor-3 (anti-FGFR-3), and the labeled cells were separated from the suspension in the magnetic field by immuno-beads coated with the second antibody. Purity of the sorted neural stem cells was found to be 93.0 %–99.0 %, with living cells amounting to 80 %–85 %. The magnetic cell sorting system could effectively separate precartilaginous stem cells from perichondrium cell suspensions.

Keywords

magnetic beads / precartilaginous stem cell (PCSC) / anti-fibroblast growth factor receptor-3 (anti-FGFR-3)

References

[1] RobinsonD., HasharoniA., CohenN., et al.. Fibroblast growth factor receptor-3 as a marker for precartilaginous stem cells. Clin Orthop, 1999, 367(Suppl): 163-75

[2] KemsheadJ. T., UgelstadJ.. Magnetic separation techniques: their application to medicine. Mol Cell Biochen, 1985, 67(1): 11-18

[3] MiltenyiS., MulerW., WeichelW., et al.. High gradient magnetic cell separation with MACS. Cytometry, 1990, 11(3): 231-238

[3a] GhosseR. A., OsmanI., BhattacharyaS., et al.. Detection of prostatic specific membrane antigen messenger RNA using immunobead reverse transcriptase polymerase chain reaction. Diagn Mol Pathol, 1999, 8(2): 59-65

[4] NishiH., NishimuraS., HigashiuraM., et al.. A new method for histamine release from purified peripheral blood basophils using monoclonal antibody-coated magnetic beads. J Immunol Methods, 2002, 240(1–2): 39-46

[5] ChosseR. A., OsmanI., BhattacharyaS., et al.. Detection of prostatic specific membrane antigen messenger RNA using immunobead reverse transcriptase polymerase chain reaction. Diagn Mol Pathol, 1999, 8(2): 59-65

This project was supported by a grant from the National Natural Sciences Foundation of China (No. 30170944).