Effects of Danshen injection on the malignant obstructive jaundice in the SD rat model

Abstract

To observe the effects of Danshen on the growth of hepatocellular carcinoma in the SD rats, a model of malignant obstructive jaundice was established by inoculation of transplanted tumor into the hepatic portal with the walker-256 hepatocarcine line, which resulted in the obstruction by the infiltration and metastasis of hepatocellular carcinoma. SD rats were divided into 4 groups: the rats were treated by 0.9 % NS (n=24, control group), inosine+vitamin C (n=40, InV group), Danshen (n=40, DS group) and 5-FU (n=40, 5-FU group), respectively. The liver function, morphological changes and the expressions of PCNA, VEGF and ICAM-1 in carcinoma foci, peri-carcinoma tissues, adjacent lobe (left-internal lobe) and lung tissues were observed after the treatment with the 4 agents. Our results showed that the protective effect of Danshen on liver function was significantly better than that of NS and 5-FU (P<0.01). No significant difference in protective effect was observed between DS group and InV group (P>0.05). Danshen also provided protective effect on the morphological damage of liver caused by obstructive jaundice. The rates of carcinoma-inhibition and metastasis inhibition were significantly higher than those of NS and inosine+vitamin C (P<0.01). No significant difference in this regard existed between DS group and 5-FU group (P>0.05). The expressions of PCNA, VEGF and ICAM-1 PCNA, VEGF and ICAM-1 in carcinoma foci, peri-carcinoma tissues, adjacent lobe (left-internal lobe) and lung tissues were lower than those in control group and InV group, with the differences being significant (P<0.01). No significant differences were found between DS group and 5-FU group in the expression levels of PCNA and VEGF (P>0.05) but ICAM-1 (P<0.05). It is concluded that Danshen injection not only has protective effects on liver injury caused by obstructive jaundice, but can inhibit the proliferation and growth of hepatocarcinoma, interfere with the vascularization of tumors, prevent recurrence and metastasis of hepatocarcineoma.

Keywords

malignant biliary obstruction model / proliferating cell nuclear antigen (PCNA) / vascular endothelia cell growth factor (VEGF) / intercellular adhesion molecular-1 (ICAM-1) / infiltration and metastasis / Danshen

References

[1] XiaR. L., LiuQ. G., NiW. D., et al.. Appraisement and the establishment of malignant obstruction jaundice model. J Xi’an Med Univ (Chinese), 2001, 22(5): 33-35

[2] YuanS. L., WangX. J., WeiY. Q.. Anticancer Effect of Taneshion and Its Mechanism. J Chin J Cancer (Chinese), 2003, 22(12): 1363-1366

[3] XiaR. L., ChenX. P.. Effect of Danshen injection on hepato-cellular apoptasis in SD rat models with malignant biliary obstruction. J Fourth Mil Med Univ (Chinese), 2006, 27(19): 1802-1804

[4] LiangY., YangY. M., YuanS. L.. Dansheng ketone medicine reason takes affect and is clinical to study progress. J Chin Herbal Med (Chinese), 2000, 31(4): 304-306