The role of sICAM-1 detection in the diagnosis of acute rejection following liver transplantation

Abstract

In order to evaluate the applied value of soluble intracellular adhesion molecule-1 (sICAM-1) in acute rejection (AR) following liver transplantation, the expression of sICAM-1 protein was sequentially detected by using ELISA in serum and bile of 43 patients receiving liver transplantation. In AR group, the expression levels of sICAM-1 protein were increased 3 days before and immediately on the establishment of AR diagnosis, and there was significant difference in the expression of bile between AR group and control group (P<0.01). After reversion of AR with hormone intensive therapy, there was significant difference in the sICAM-1 protein expression of serum and bile between AR group and control group. It was concluded that the sequential detection of sICAM-1 protein level in serum and bile was a reliable and noninvasive method for the early diagnosis of AR after liver transplantation and was valuable to observe the curative effects of anti-AR therapy.

Keywords

orthotopic liver transplantation / acute rejection / bile / serum / sICAM-1

References

[1] AdamsD. H., MainolfiE., EliasE., et al.. Detection of circulating intercellular adhesion molecule-1 after liver transplantation—evidence of local release within the liver during graft rejection. Transplantation, 1993, 55(1): 83-87

[2] FuY. Y., HeX. S., ChenJ. L., et al.. Expression of sICAM-1 in rat bile and its diagnostic value in acute liver rejection. J Hepatobil Surg (Chinese), 2001, 9(1): 66-68

[3] NinovaD., KromR. A., WiesnerR. H.. Hepatic allograft rejection is associated with increased levels of soluble intercellular adhesion molecule-1. Liver Transpl Surg, 1995, 1(5): 290-295

[4] WarleM. C., MetselaarH. J., HopW. C., et al.. Early differentiation between rejection and infection in liver transplant patients by serum and biliary cytokine patterns. Transplantation, 2003, 75(1): 146-151

[5] WangZ. J., ChenG. H., HeX. S., et al.. Significance of gene expression of inerleukin-6 and interferon-γ in bile for diagnosis of acute rejection after liver transplantation in rats. Chin J Hepatobil Surg (Chinese), 2003, 9(9): 550-553

[6] LangT., KramsS. M., VillanuevaJ. C., et al.. Differential patterns of circulating intercellular adhesion molecule-1 (cICAM-1) and vascular cell adhesion molecule-1 (cVCAM-1) during liver allograft rejection. Transplantation, 1995, 59(4): 584-589

[7] AdamsD. H., HubscherS. G., ShawJ., et al.. Intercellular adhesion molecule 1 on liver allografts during rejection. Lancet, 1989, 2(8672): 1122-1125

[8] MarteliusT., SalmiM., WuH., et al.. Induction of vascular adhesion protein-1 during liver allograft rejection and concomitant cytomegalovirus infection in rats. Am J Pathol, 2000, 157(4): 1229-1237