Flow cytometry DNA measurement for prediction of effect of therapy in acute leukemia

Abstract

We measured the DNA content of bone marrow (BM) aspirate by flow cytometry (FCM) in 41 cases of acute leukemia (AL) and found no remarkable relation between pretreatment DNA index and percentage of proliferative cells and prognosis (P>0.05). After chemotherapy, reduction of proliferative cells rate, disappearance of DNA aneuploidy and decrease of leukemic cells in BM could be considered as the reliable indicators of effective treatment. Complete remission (CR) rate was significantly higher in patients responsive to treatment (81.82 %) than in those unresponsive (22.22 %; P< 0.05). It seems that these parameters may serve as indicators of efficiency of chemotherapy and be useful in monitoring regimen in time and in reducing the occurrance of chemotherapy-resistance, thereby facilitating individualization of chemotherapy in AL patients.

Keywords

DNA measurement / flow cytometry / acute leukemia / chemotherapy / efficiency

References

[1] MaddoxAM, et al. . DNA-RNA measurements in patients with acute leukemia undergoing remission induction therapy. J Clin Oncol, 1985, 3(6): 799-808

[2] GrayJM, et al. . GrayJM, DarzynkiewiczZ, et al. . Flow cytokinetics. Techniques in Cell Cycle Analysis, 1987, New Jersey, Humana Press Clifton: 93-137

[3] TaylorLW, et al. . A rapid single step staining technique for DNA analysis by microfluorimetry. J Histochem Cytochem, 1980, 28: 1021-4

[4] DeanPN, et al. . ShapiroHM, et al. . Methods of data analysis in flow cytometry, 1985, New York Liss, Practical Flow Cytometry: 64-77

[5] HiddemannW, et al. . Convention on nomenclature for DNA cytometry. Cytometry, 1984, 5: 445-6

[6] ClarkR, et al. . Prognostic importance of hypodiploidy hemopoietic precursors in myelodysplastic syndromes. New Engl J Med, 1986, 314: 1472-5

[7] AndreeffM, et al. . Buchner, et al. . Multiparameter flow cytometry for determination of ploidy, proliferation and differentiation in acute leukemia: treatment effects and prognostic value. Tumor Aneuploidy, 1985, Berlin, Heidelberg, Springer-Verlag: 81-105

[8] DosikGM, et al. . Pretreatment flow cytometry of DNA content in adult acute leukemia. Blood, 1980, 55: 474-82

[9] BarlogieB, et al. . DNA aneuploidy in adult acute leukemia. Cancer Genet Cytogenet, 1987, 28(2): 213-28

[10] AndreeffM, et al. . Discrimination of Human Leukemia subtypes by flow cytometric analysis of cellular DNA and RNA. Blood, 1980, 55(2): 282-93

[11] BarlogieB, et al. . Cellular DNA content as a marker of neoplasia in man. Am J Med, 1980, 69: 195-203

[12] BarlogieB, et al. . Flow cytometry in clinical cancer research. Cancer Res, 1983, 43: 3982-97

[13] HiddemannW, et al. . Buchner, et al. . Incidence and heterogeneity of DNA aneuploidy in solid tumors and acute leukemia, 1985, Berlin, Heidelberg, Tumor Aneuploidy. Springer-Verlag: 71-9

[14] LookA, et al. . Aneuploidy and percentage of S-phase cells determined by flow cytometry correlate with cell phenotype in childhood acute leukemia. Blood, 1982, 60: 959-67

[15] AndreeffM, et al. . Cell kinetics of leukemia. Seminars Hematology, 1986, 23(4): 300-14

[16] HiddemannW, et al. . Cell kinetics in acute leukemia: a critical reevaluation based on new data. Cancer, 1982, 50: 250-8

[17] Andreeff M, ed. Clinical Cytometry. Annals of the New York Academy of Science 1986:387–406.

[18] BaccaraniM, et al. . Cell kinetics effect of low dose arabinosyl cytosine. Br J Haematol, 1987, 67: 33-7

[19] Drenthe-SchonkAM, et al. . Cytokinetic changes after cytosine arabinoside in acute non-lymphocytic leukemia. Leuk Res, 1981, 5(1): 89-96

[20] PreislerHD, et al. . Prediction of response of acute nonlymphocytic leukemia to therapy with high dose cytosine arabinoside. Br J Haemat, 1984, 58: 19-32

[21] BaischH, et al. . A comparison of mathematical methods for the analysis of DNA histograms obtained by flow cytometry. Cell Tiss Kinet, 1982, 15: 235-249