Observation on electrochemical detection method for diagnosis of bacteriuria

Abstract

The aim of the present work is to detect the presence of bacteriuria by measuring voltage of urine with electrochemical method. 100 specimens of urine were collected from normal persons, to 49 of which bacteria were added to obtain bacteriuria (concentration of Bacillus coli, Bacillus aerogenes and Bacillus proteus is 10⁵/ml, respectively).

The voltage of normal urine ranged from −290 mV to −471 mV, with a mean value of −394.9 mV (SD=38.3 mV), while that of bacteriuria ranged from +266 mV to −375 mV with a mean value of −80.36 mV (t=5.85,P < 0.0l). Taking −318 mV as critical line, 96% of normal urine specimens were below this voltage, with only 4% appearing false positive, while 95.92% of bacteriuria specimens were above that line, with 4.08% appearing to be false negative. The above data revealed that electrochemical detection method is valuable for distinguishing normal urine from bacteriuria. Moreover, the instrument used is simple and works with rapid and effective results.

Keywords

electrochemical method / urine voltage / bacteriuria diagnosis

References

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