Immunohistochemical demonstration of alpha-1-antitrypsin in renal tubular epithelia in Patients with or without alpha-1-antitrypsin deficiency

Abstract

Two patients with protease inhibitor (Pi) Z-, four with Pi MZ and three Pi MM patients were studied at autopsy to demonstrate alpha-1-antitrypsin (AAT) in renal tubules. Sections of liver and kidney were stained for AAT by indirect immuno-peroxidase method. All of the Pi MM patients showed strong reaction in most of the proximal and some of the distal tubular epithelia. Of six cases with abnormal Pi, one failed to give any staining, four showed positive reaction in some of the proximal tubules, one case with higher AAT in the serum than the other cases revealed relatively strong activity in most of the proximal tubules. The results suggest that AAT is frequently present in the renal tubular epithelia, but whether it is produced in that site or secondarily absorbed from glomerular filtrate is unclear.

Keywords

alpha-1-antitrypsin / immunohistochemistry / renal tubular epithelium / alpha-1-antitrypsin deficiency

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