The expression of toll-like receptor 2 and 4 mRNA in local tissues of model of oropharyngeal candidiasis in mice

Abstract

To investigate the expression of Toll-like receptor (TLR) 2 and 4 mRNA in local tissues of model of oropharyngeal candidiasis in mice and to explore the potential role of TLR2 and TLR1 in carlier period of immune response, a murine model of oropharyngeal candidiasis inoculated by cotton wool balls saturated withcandida albicans was established. Mice were sacrificed at the indicated time points and the oropharyngeal tissues were excised. The expression of TLR2 and TLR4 mRNA was detected by RT PCR. The results showed that low level of TLR2. 1 mRNA could be detected in oropharyngeal tissues, but they were markedly up-regulated 6 h after inoculation, peaking after 12⁻¹ 24 h. Tissue TLR4 mRNA was gradually down-regulated 21⁻ 48 h, while TLR2 mRNA levels remained high up to the 72nd h. These data suggested that oropharyngeal infection ofCandida albicans could result in up-regulation of TLR2 1 mRNA expression in local tissues, which might play important roles in earlier period of immune response.

Keywords

Candida albicans / oropharyngeal infection / Toll like receptor

References

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