Studies on cellular mechanism of the antishock action of anisodamine in rats—Effect of anisodamine on the stability of liver lysosomes and comparison of its membrane-stabilizing action with that of dexamethasone

Abstract

In rat hemorrhagic shock, we found that like dexamethasone, anisodamine (654-2) might increase the stability of hepatic lysosomes and improve the prognosis of shock. In vitro experiments showed that anisodamine had no direct stabilizing effect on isolated hepatic lysosomal granules from shocked rats probably due to its mechanism of action different from steroid. Experiments with isolated perfused liver exhibited that 654–2 could abate the labilization of lysosomes caused by hypoxia. From the above results it can be inferred that anisodamine might have a direct cell protection effect on the hypoxic or ischemic liver, and the protective action of 654–2 on lysosomes during shock is a consequence of its cell protection action.

Keywords

hemorrhagic shock / hepatic lysosomes / cathepsin D / anisodamine / dexamethasone / cell protection

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