Normal and leukeymic lmphocyte membrane fluidity and response to stimulation with ConA and PHA

Abstract

In our experiment, lymphocyte membrane was 1abeled by DPH fluorescence probe. The rate of rotation of the probe can be measured from the value of fluorescence polarization (P_DPH). With this method useful information could be provided about membrane fluidity of lymphocytes. It was found that the F value (unit of lipid fluidity of membrane) of leukemic lymphocytes was obviously higher than that of normal ones. Furthermore, the F value of cultured leukemic T_s lymphocytes was the highest. In contrast with normal spleen T-lymphocytes or mixed lymphocytes, the response of malignant lymphocytes to the stimulation with ConA or PHA was reflected in the decrease of P_DPH value or the increase of F value. Unexpectedly, the F value of T-lymphocytes from “615” mouse not injected with tumour cells was also higher than that of the mixed. The possibility of using the membrane fluidity as a diagnostic criterion was also discussed.

Keywords

fluorescence polarization / membrane of lymphocytes / membrane fluidity / T / lymphocytic leukemia / ConA / PHA

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