Studies on biological characteristics and modulating factors of long-term cultured bone marrow cells of a myeloma patient in vitro

Abstract

In the present study we cultured in vitro bone marrow cells from a patient with multiple myeloma (IgD) and researched into the modulation of supernatant media of various leukemic lines on the growth of culture cells. Cells in the cultures were studied for their morphological, biochemical, immunological and ultrastructural features. Drug sensitivity assay was also performed. The results showed that supernatant media of 6 cell lines promoted cell growth, but most remarkable stimulating activity was displayed by supernatant media from U937 and CEM. Cell cloning effect attained to more than 90 %. Cultured cells possessing biological characteristics of malignant cells were probably malignant cells from B cell lineage. This study indicated that long-term cultures of marrow cells might provide a tool useful for clinical and laboratory purpose and a method for studies on pathogenesis, regulation of hematopoiesis, cell differentiation and guide-way drugs.

Keywords

cell culture / conditioned medium / cell biological characteristics / monoclonal antibody / drug screening

References

[1] BurgessAW, et al. . Stimulation by human placental conditioned medium of hemopoietic colony-formation by human marrow cells. Blood, 1977, 49: 573-83

[2] MetcalfD, et al. . Responsiveness of human granulocytic leukemic cells to colony-stimulating factor. Blood, 1974, 43: 847-59

[3] cAMP, cGMP 1989; 5 (l):29–31.

[4] 1988;4(5):299–301.

[5] HarrisPE, et al. . Distinct differentiationinducing activities of γ-interferon and cytokine factors acting on the human promyelocytic leukemia cell line HL-60. Cancer Res, 1985, 45: 3090-5

[6] CSF 1982; 3(1): 1–4.