The effects of cadmium, mercury and lead in vitro on hepatic microsomal mixed function oxidase and lipid peroxidation

Abstract

In this study, the authors investigated the effect of cadmium, mercury and lead in vitro on hepatic microsomal mixed function oxidase and lipid peroxidation. The results showed that these metals could inhibit the activity of hepatic microsomal aniline hydroxylase, decrease the concentration of hepatic microsomal cytochrome P-450 and increase the concentration of the inactive form of hemoprotein, P-420. Besides, they could enhance hepatic microsomal lipid peroxidation. There were marked concentration-dependent responsesin these microsomal reactions. Thus, it may be suggested that cadmium, mercury and lead are capable of impaizing hepatic microsomal mixed function oxidase in vitro by stimulating membrane lipid peroxidation.

Keywords

cadmium / mercury / lead / cytochrome P-450 / lipid peroxidation

References

[1] HruszkewyczAM, et al. . Destruction of microsomal cytochrome P-450 and glucose-6-phosphatase by lipid extracted from peroxidized microsomes. Toxicol Appl Pharmacol, 1978, 46: 695-695

[2] CadenasE, et al. . Low level chemiluminescence of liver micorsomal fractions initiated by tert-butyl hydroperoxide. Eur J Biochem, 1982, 124: 349-349

[3] XiaoGH, et al. . Effects of some toxic metals on microsomal oxidase system of the rat. Acta Acad Med Wuhan, 1984, 4: 158-62

[4] YonahaM, et al. . Effects of trivalent and hexavalent chromium on lipid peroxidation in rat liver microsomes. Chem Pharm Bull, 1980, 28: 893-893

[5] StaceyNH, et al. . Cadmium toxicity and lipid peroxidation in isolated rat hepatocytes. Toxicol Appl Pharmacol, 1980, 53: 470-470

[6] StaceyNH, KappusH. Cellular toxicity and lipid peroxidation in response to mercury. Toxicol Appl Pharmacol, 1982, 63: 29-29

[7] 1985; 3(4):157.

[8] OmuraT, et al. . The carbon monoxide-binding pigment of liver microsomes. J Biol Chem, 1964, 239: 2379-2379

[9] O’BrienPJ, et al. . ColowickSP, KaplanNO, FleischerS, PacketL, et al. . A peroxidase assay for cytochrome P-450. Methods in Enzymology, 1978, New York, Academic Press Inc.: 407-12

[10] StaceyNH, et al. . Cadmium toxicity and lipid peroxidation in isolated rat hepatocytes. Toxicol Appl Pharmacol, 1980, 53: 470-470

[11] LowryOH, et al. . Protein measurement with the Folin phenol reagent. J Biol Chem, 1951, 193: 265-265

[12] MimnaughEG, et al. . The effects of adriamycin in vitro and in vivo on hepatic microsomal drug-metabolizing enzymes: role of microsomal lipid peroxidation. Toxicol Appl Pharmacol, 1981, 61: 313-313

[13] HrycayEG, et al. . Cytochrome P-450 as a microsomal peroxidase utilizing a lipid peroxide substrate. Arch Biochem Biophys, 1971, 147: 14-14

[14] JefferyE, et al. . Effects of linoleic acid hydroperoxide on the hepatic monooxygenase systems of microsomes from untreated, phenobarbital-treated and 3-melhylcholanthrene-treated rats. Mol Pharmacol, 1977, 18: 415-415