Experimental study of plasmid TGF-β1 DNA gene transfer with lipofectamine into rabbit corneal epithelial cellsin vitro

Abstract

To investigate whether the TGF-β1 plasmid DNA carried by lipofectamine could be introduced into cultured rabbit corneal epithelial cells, specific expression of the plasmid pMAM TGF-β1 in the cultured corneal epithelial cells was studied. Two days after 12 h of transfection of pMAMT-GF-β1 mediated by lipofectamine into the cultured corneal epithelial cells, the TGF-β1 protein expression specific for pMAMTGF-β1 in the cells was detected by means of immunohistochemical staining and the positive rate was 23.37%. The results suggested that foreign plasmid DNA could be effectively delivered into cultured rabbit corneal epithelial cells by means of lipofectamine, and this will provide a promising method of studying TGF-β1 on the mechanism of physiology and pathology concerned with corneal epithelial cells.

Keywords

cornea / epithelial cells / gene transfer / transforming growth factor beta

References

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