Transfection of articular chondrocytes with rhBMP7 gene and its expression

Abstract

In order to investigate the possibility of expression of exogenous gene in transduced articular chondrocytes, plasmid pcDNA3-rhBMP7 was delivered to cultured chondrocytes. Through immunohistochemical staining and RT-PCR assay, the expression of rhBMP7 gene was detected. And the bioactivity of transgene expression product was detected through MTT assay as well. It was confirmed that exogenous gene could be expressed efficiently in transduced chondrocytes and the transgene therapy on the problems of articular cartilge.

Keywords

transfection / articular chondrocytes / gene / bone morphogenetic protein-7

References

[1] GrandeD A, BreibartA S, MasonJ M, et al. . Cartilage tissue engineering: current limitations and solutions. Clin Orthop, 1999, 367S: 176-176

[2] CookS D, RuegerD C. Osteogenic protein-1: biology and application. Clin Orthop, 1996, 324: 29-29

[3] GoldringM B. Skeletal tissue response cytokines. Clin Orthop, 1990, 258: 245-245

[4] EvansC H, RobbinsP D. Current concepts review. Possible orthopaedic applications of gene therapy. J Bone and Joint Surg, 1995, 77A: 1103-1103