Effects of 3, 4-Dihydroxyacetophenone on hypoxic vasoconstriction in isolated pulmonary and basilar arterial rings

Abstract

The effects of 3, 4-DHAP on hypoxic vasoconstriction response in pulmonary (PA) and basilar arterial (BA) rings of rabbits and their mechanism were compared in vitro.

3,4-DHAP in different concentration (2. 64 × 10^-4,7. 92 × 10^-4,2. 376 × 10^-3 mol/L) decreased the basal tone of PA rings by 32. 39 ± 9. 4 mg, 68. 96± 26. 54 mg and 145. 60 ± 58. 07 mg respectively, while the tension of the BA rings was decreased by 13. 80 ±5. 08 mg, 17.18 ± 3. 36 mg and 25. 00 ± 4. 02 mg respectively. In PA rings it also decreased the percentage increase in tension induced by hypoxia (TIH%) from the control value 48.82 ± 5.75% to 10.02 ± 3.62%, 2. 14 ± 0.96%, and 0. 00 % respectively, while in BA rings from 27. 27 ± 5.78% to 11. 23 ± 2.71%, 7.49 ± 1.62%, and 1.45 ± 1.13% respectively. The effects of 3, 4-DHAP on TIH% were partially blocked by indomethacin 10 M and L-NAME 10 M.

The results showed that 3, 4-DHAP can decrease the hypoxic pulmonary and basilar vasoconstriction in vitro, which can be partially inhibited by cyclooxygenase inhibitor and NO/EDRF inhibitor.

Keywords

pulmonary artery / basilar artery / hypoxia / 3, 4 Dihydroxyacetophenone

References

[1] ArcherS L, TolinsJ P, WeirE K. Hypoxic pulmonary vasoconstriction is enhanced by inhibition of synthesis of the endothelium derived relaxing factor. Biochem Biophys Res Commun, 1987, 164: 1198-1198

[2] 1977; 8: 7

[3] BrownI P, ThompsonC I, BelloniF L. Role of nitric oxide in hypoxic coronary vasodilation in isolated perfused guinea pig heart. Am JPhysiol, 1993, 264: H821-H821

[4] CocksT M, AngusJ A. Endothelium dependent relaxation of coronary arteries by NE and Serotonin. Nature, 1983, 305: 627-627

[5] 3,4-1981; 2(2);107–110.

[6] GleasonC A, HammC, JonesM D, et al. . Effect of acute hypoxaemia on brain blood flow and oxygen metabolism in immature fetal sheep. Am JPhysiol, 1990, 258: H1064-H1064

[7] HaughtW H, YangB C, LawsonD L, et al. . Slow channel calcium blocker attenuates reoxygenation mediated vascular contraction. J Cardiovasc Pharmacol, 1992, 19: 701-701

[8] HoldenW E, McCallE. Hypoxia induced contraction of porcine pulmonary artery strips depend upon intact endothelium. Exp Lung Res, 1984, 7: 101-101

[9] 1993; 28: 333

[10] JinN, PackerC S, RhoadesR A. Pulmonary arterial hypoxic signal transduction. Am J Physiol, 1992, 263: L73-L73

[11] JinX R, FanM, WangZ Q, et al. . Strain differences in pulmonary vascular responsiveness to hypoxia in rats. J Tongji Med Univ, 1990, 10: 134-134

[12] UshaJ. Rajand Prscilla C. Role of eicosanoids in hypoxic vasoconstriction in isolated Lamb lungs. Am J Physiol, 1987, 251: H626-H626

[13] KatusicZ S, VanhoutteP M. Anoxic contraction in canine cerebral contraction in isolated canine cerebral arteries; contribution of endothelium derived factors, metabolites of arachidonic acid, and calcium entry. J Cardiovasc Pharmacol, 1986, 8(8): S97-S97

[14] KovitzK L, AleskoT D W, SylvesterJ T, et al. . Endothelium derived relaxing and contracting factors contribute to hypoxic response of PA. Am J Physiol, 1993, 265: H1139-H1139

[15] 1993; 22: 91

[16] MartinD, BarnesS D, WetzelR D. Acute hypoxia alters eicosanoids production of perfused PA endothelial cells in culture. Prostaglandins, 1992, 43: 371-371

[17] MC MurtryI F, DavidsonA B, ReevesJ T, et al. . Inhibition of hypoxic pulmonary vasoconstriction on calcium antagonists in isolated rats lungs. Circ Res, 1976, 38: 99-99

[18] NuramatsuM, ShimizuL, AssanoH, et al. . Hypoxia elicited contraction of aorta and coronary artery via removal of EDRF. Am J Physiol, 1992, 263: H1339-H1339

[19] TsengC M, GoodmanL W, RubinL J, et al. . N G. monomethyl-L-arginin paradoxically relaxes pre-contracted canine intrapulmonary arteries. J Appl Physiol, 1993, 74: 549-549

[20] WangD X, JinX R, WangW H, et al. . Effects of cigarette smoking on HPV and its relation to animal species and period of smoking. J Tongji Med Univ, 1992, 12: 75-75

[21] WeirE K, McMurtryX, TuckerA, et al. . Prostaglandin synthesis inhibitors do not decrease hypoxic pulmonary vasoconstriction. J Appl Physiol, 1976, 41: 714-714

[22] YangD S. Effects of 3, 4-Dihydroxyacetophenone on the biosynthesis of TXA and PGI in human placental villus and umbilical artery segments in vitro. Prostaglandins, 1989, 38: 497-497