Study on lymphocyte activation and proliferation induced by anti-CD3 McAb

Li Ming; Yang Jing; Shen Guan-xin; Zhang Qian; Liu Shen-pei; Liu Zhong-bei; Ye Wei-xin

doi:10.1007/BF02897669

Current Medical Science ›› 1994, Vol. 14 ›› Issue (4) : 209 -212. DOI: 10.1007/BF02897669

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Study on lymphocyte activation and proliferation induced by anti-CD₃ McAb

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Abstract

T cell activation and proliferation via CD₃-TCR complex were investigated by lymphocyte DNA synthesis in vitro. Several interfering factors were also discussed. The result indicated that lymphocyte activation and proliferation are calciumdependent. A rise of cytoplasmic free Ca²⁺ quickly following activation with CD₃ McAb is mainly due to intracellular mobilization of Ca²⁺, while lymphocyte proliferation needs both intracellular mobilization of Ca²⁺ as well as influx of extracellular Ca²⁺. It was confirmed that CTX sensitive G protein plays a role in regulating T cell proliferation by pretreatment with CTX suppressing lymphocyte³H-TdR incorporation obviously. PLC and PKC inhibitor neomycin and P. S. S could also decrease T cell proliferation.

Keywords

CD₃ McAb / lymphocyte activation and proliferation / GTP-binding protein / cytoplasmic free calcium

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Li Ming, Yang Jing, Shen Guan-xin, Zhang Qian, Liu Shen-pei, Liu Zhong-bei, Ye Wei-xin. Study on lymphocyte activation and proliferation induced by anti-CD₃ McAb. Current Medical Science, 1994, 14(4): 209-212 DOI:10.1007/BF02897669

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