Expression of tenascin and fibronectin in nasal polyps

Abstract

To explore the role of tenascin (TN) and fibronectin (FN) in the pathophysiology of nasal polyps (NP), the expression of TN and FN in NP from 34 patients and inferior turbinates from 20 patients with deviation of nasal septum was immunohistochemically studied. In patients with NP, the relations between expression and histopathological features, eosinophils (EOS) infiltration, clinical staging and the size of NP were analyzed. Our study showed that the gray score of TN and FN expression was 163.10±10.54 and 163.24±11.52 in NP respectively, whereas it was 175.49±9.29 and 173.93±7.92 in inferior turbinates respectively. The difference between two groups was significant (P<0.01). The expression of TN and FN in edematous type was significantly stronger than that in cystic and glandular type and fibrous type (P<0.05). The association between FN expression and EOS infiltration was significant (r=−0.60,P<0.01). The expression of TN and FN did not correlate with clinical staging and size (P>0.05). It was suggested that abnormal ECM might contribute to proliferation of epithelia, accumulation of EOS and edema formation, thereby causing development of NP.

Keywords

nasal polyps / extracellular matrix / fibronectin / tenascin

References

[1] WangQ P, StudierE, Roudot-ThoravalF, et al. . Myofibroblast accumulation induced by transforming growth factor-β₁ is involved in the pathogenesis of NP. Laryngoscope, 1997, 107: 926-926

[2] Caye-ThomasenP, HermanssonA, TosM, et al. . Polyp pathogenesis — a histopathological study in experimental otitis media. Acta Otolaryngol (Stockh), 1995, 115: 76-76

[3] 1998,33:134

[4] MalmL. Assessment and staging of NPosis. Acta Otolaryngol (Stockh), 1997, 117: 465-465

[5] KakoiH, HiraideF. A histological study of formation and growth of NP. Acta Otolaryngol (Stockh), 1987, 103: 137-137

[6] RennardS I. Inflammation and repair process in chronic obstructive pulmonary disease. Am J Respir Crit Care Med, 1999, 160: 812-812

[7] NakagawaT, YamaneH, NakaiY, et al. . Comparative assessment of cell proliferation and accumulation of extracellular matrix in NP. Acta Otolaryngol (Stockh), 1998, 538(Suppl): 205-205

[8] HerardA L, PierrotD, HinnraskyJ, et al. . Fibronectin and its alpha 5 beta 1-intergrin receptor are involved in the wound repair process of airway epithelium. Am J Physiol, 1996, 271: 2726-2726

[9] TrembleP, Chiquet-EhrismannR, WerbZ. The extracellular matrix ligands fibronectin and tenascin collaborate in regulating collagenase gene expression in fibroblasts. Mol Biol Cell, 1994, 5: 439-439

[10] 1999, 33: 353

[11] LeeS H, ParkJ H, OhB H, et al. . Analysis of proteoglycan gene messages in human nasal mucosa and NP using dot blot hybridization. Acta Otolaryngol (Stockh), 2001, 121: 398-398

[12] SeminarioM C, BochnerB S. Expression and function of beta 1 intergrins on human eosinophils. Mem Inst Oswaldo Cruz, 1997, 92(suppl2): 157-157