Effects of arecoline on calcium channel currents and caffeine-induced calcium release in isolated single ventricular myocyte of guinea pig

Xianming Lin; Zhen Li; Benrong Hu; Guojin Xia; Weixing Yao; Jizhou Xiang

doi:10.1007/BF02896763

Current Medical Science ›› 2002, Vol. 22 ›› Issue (4) : 279 -280. DOI: 10.1007/BF02896763

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Effects of arecoline on calcium channel currents and caffeine-induced calcium release in isolated single ventricular myocyte of guinea pig

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Abstract

The effects of Arecoline (Are) on calcium mobilization were investigated. In isolated single ventricular myocyte of guinea pig, patch clamp whole cell recording techniques were used to record the current of L-type calcium channel and cytosolic Ca²⁺ level (Ca²⁺i) labeled with fluorescence probe Fluo-3/AM was measured under a laser scanning confocal microscope. Results revealed that Are (3–100 µmol/L) could inhibit L-type calcium current in a concentration-dependent manner and the value of IC₅₀ was 33.73 µmol/L (n=5). In the absence of extracellular calcium, the resting levels of [Ca²⁺]i was not affected by Are (n=6,P>0.05), but pretreatment with Are (30 µmol/L) could significantly inhibit the [Ca²⁺]i elevation induced by caffeine (10 mmol/L,n=6,P<0.01). It was concluded that Are could inhibit not only calcium influx through L-type calcium channel but also calcium release from sarcoplasmic reticulum.

Keywords

arecoline / patch clamp / calcium channel / laser scanning confocal microscope / cytosolic Ca²⁺ level

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Xianming Lin, Zhen Li, Benrong Hu, Guojin Xia, Weixing Yao, Jizhou Xiang. Effects of arecoline on calcium channel currents and caffeine-induced calcium release in isolated single ventricular myocyte of guinea pig. Current Medical Science, 2002, 22(4): 279-280 DOI:10.1007/BF02896763

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