Whole CagA gene amplification ofhelicobacter pylori and its fingerprinting by restriction fragment length polymorphism

Siying Ye; Jienan Ao; Ying Peng; Haifeng Yue; Fang Liao; Guoping Hu; Yang Xu; Zhengmao Zhang

doi:10.1007/BF02896762

Current Medical Science ›› 2002, Vol. 22 ›› Issue (4) : 276 -278. DOI: 10.1007/BF02896762

Article

Whole CagA gene amplification ofhelicobacter pylori and its fingerprinting by restriction fragment length polymorphism

Author information +

History +

PDF

Abstract

To set up a method of amplification for the whole CagA gene ofHelicobacter pylori and its fingerprinting by restriction fragment length polymorphism (RFLP), nested PCR was employed in combination with TD-PCR to amplify the gene and EcoRI and Hind III were used to generate the RFLP fingerprinting. Target DNA fragments from 13 of 20 samples were successfully amplified and the relevant RFLP fingerprintings were obtained. It is concluded that the method can be used to amplify the whole CagA gene and CagA gene has apparent diversity of RFLP profile.

Keywords

Helicobacter pylori / cagA / PCR / RFLP

Cite this article

Download citation ▾

Siying Ye, Jienan Ao, Ying Peng, Haifeng Yue, Fang Liao, Guoping Hu, Yang Xu, Zhengmao Zhang. Whole CagA gene amplification ofhelicobacter pylori and its fingerprinting by restriction fragment length polymorphism. Current Medical Science, 2002, 22(4): 276-278 DOI:10.1007/BF02896762

登录浏览全文

4963

注册一个新账户忘记密码

References

Publishing order | Descend order by publishing year | Descend order by cited within

[1]	LeunkR D, JihismP T, DavidB C, et al. . Cytotoxin activity in broth culture filtrates of Campylobacter pylori. J Med Anirobroh, 1998, 16: 93-93

[2]	CoverT I, DooleyC P, BlaserM J, et al. . Characterization of human serologic to proteins inHelicobacter pylori broth culture supernatants with vacuolizing cytotoxin activity. Infect Immun, 1990, 58: 603-603

[3]	CrabtreeJ E, CovacciA, FarmerysS M, et al. . Helicobacter pylori induced interleukin-8 expression in gastric epithelial cells is associated with CagA positive phenotype. J Clin Pathol, 1995, 48: 41-41

[4]	EgalE D, ChaJ, LoJ, et al. . Altered states: Involvement of phosphorylated CagA in the induction of host cellular growth changes byHelicobacter pylori. Proc Natl Acad Sci USA, 1999, 96: 14559-14559

[5]	HigashiH, TsutsumiR, MutoS, et al. . SHP-2 tyrosine phosphatase as an intracellular target ofHelicobacter pylori CagA protein. Science, 2002, 295(5555): 683-683

[6]	CoverT L, GluczynskiY, LageA P, et al. . Serological detection of infection with cagAHelicobacter pylori strains. J Clin Microbiol, 1995, 33: 1496-1496

[7]	YamaokaY, KodamKaT, KashimaK, et al. . Vatiants of the 3′ region of the cagA gene inHelicobacter pylori isolates from patients with different H. pylori associated diseases. J Clin Microbiol, 1998, 36: 2258-2258