Effect of HDL and apoAI on PGE₂ production by monocyte-derived macrophages

Abstract

Effect of antiatherogenic high density lipoprotein (HDL) and apolipoprotein AI (apoAI) on production of prostaglandin E₂(PGE₂) by human monocyte-derived macrophages was investigated. Macrophages were loaded with acetylated low density lipoprotein followed by incubation with HDL₃ or apoAI. PGE₂ produced and secreted in culture supernatant was quantified by enzyme immunoassay. HDL₃ induced production of PGE₂ by macrophages in a time-dependent manner. 24 h after incubation, PGE₂ production by HDL₃-treated macrophages increased 3. 7-fold of that by control cells. ApoAI also induced PGE₂ secretion to 2. 1-fold, which was significantly less than HDL₃. The data indicate that both HDL₃ and lipid-free apoAI enhance PGE₂ synthesis and secretion by human macrophages and this may further contribute to the protection from atherosclerosis.

Keywords

prostaglandin E₂ / high density lipoprotein / macrophage / monocyte-derived / apolipoprotein AI / atherosclerosis