Susceptibility to vaginal candidiasis under different conditions in mice

Abstract

In order to study the susceptibility of murine vaginal mucosa to Candida albicans under different conditions, vaginal lavage fluid and vaginal tissue of mice were observed and compared between murine models with normal immune system (estrogen-treated mice) and immunosuppressed murine model, and between primary infection model of vaginal candidiasis and secondary infection one. The average level of colony forming unit (CFU) from the immunosuppressed group was higher than that from estrogen-treated group at each time point and the peak time was delayed. The differences between the two groups were statistically significant (P<0.05) from the fourths day after inoculation. A significant difference existed in the average level of CFU between the control group and the estrogen-treated group (P<0.05), and between the control group and the immuosuppressed group (P<0.01). It was concluded that the vaginal mucosa from the immunosuppressed mice is more susceptible to Candida albicans and no difference is found in susceptibility between mice with primary infection and secondary infection.

Keywords

Candida albicans / vaginal candidiasis / susceptibility / mice

References

[1] SobelJ D. Pathogenesis and epidemiology of vulvovaginal candidiasis. Ann. N Y Acad Sci, 1988, 544: 547-547

[2] HaefnerH K. Current evaluation and management of vulvovaginitis. Clin Obster Gynecol, 1999, 42(2): 184-184

[3] ZhaoJ J, ShenY N, ChenW, et al. . A murine model of candida albicans vaginitis. Chin J Dermatol (Chinese), 2002, 35(5): 346-346

[4] HachisukaH, SasaiY. Development of experimental sporotrichosis in normal and modified animals. Mycopathologia, 1981, 76(2): 79-79

[5] FidelP L, WolfN A, KuKurugaM A. T lymphocytes in the murine vaginal mucosa are phenotypically distinct from those in the periphery. Infect Immun, 1996, 64(9): 3793-3793

[6] HeidenreichS, KubisT, SchmidtM, et al. . Glucocorticoid-induced alterations of monocyte defense mechanisms against Candida albicans. Cell Immunol, 1994, 157(2): 320-320

[7] PiemontiL, MontiP, AllavenaP, et al. . Glucocorticoids affect human dendritic cell differentiation and maturation. J Immunol, 1999, 162: 6473-6473

[8] MoserM, De SmedtT, SornasseT, et al. . Glucocorticoids down-regulate dendritic cell functionin vitro andin vivo. Eur J Immunol, 1995, 25: 2818-2818

[9] WozniakK L, WormleyF L, FidelP L, et al. . Candida-Specific Antibodies during Experimental Vaginal Candidiasis in Mice. Infect Immun, 2002, 70(10): 5790-5790

[10] FidelP L, CutrightJ, SteeleC. Effects of Reproductive Hormones on Experimental Vaginal Candidiasis. Infect Immun, 2000, 68(2): 651-651