Effect of cyclosporin a eye drop on keratoconjunctivitis sicca and its mechanism

Abstract

In this study, the effect of cyclosporin A (CsA) eye drop on keratoconjunctivitis Sicca (KCS) and its mechanism were studied. The KCS models were established by injecting Pertussis vaccine, complete freund's adjuvant (CFA) and antigen of conjunctiva from isotype mice. Then the KCS models were treated with cyclosporin A eye drop. Changes in breaking-up time (BUT), lacrimal secretion in 30 min and diversion in 24 h were measured. The percentage of beaker cells, the lymphocytic infiltration in conjunctiva were observed. The expression levels of Aquaporin-3 (AQP3) in conjunctiva epithelial cells, beaker cells and accessory lacrimal gland were immunohistochemically detected. The results showed that there were significant differences in BUT, the percentage of beaker cells, lacrimal secretion in 30 min, the lymphocytic infiltration and the expression of AQP3 between the experimental group and an control group. It was concluded that CsA eye drop exerts marked therapeutic effect on KCS by inhibiting T lymph cells, increasing the goblet cells and AQP3 expression in conjunctiva.

Keywords

keratoconjunctivitis sicca / cyclosporin A eye drop / beaker cell / aquaporin-3

References

[1] DeanD H, HiramotoR N. Experimental autoallergic sialodenitis in male rats. J Oral Pathol, 1984, 13(1): 63-63

[2] KondoE, MatsuiH, MatsuoY, et al. . Novel function of Calcineurin-multipotential factor as protein a phosphatase. Nippon Rinsho, 1998, 56(11): 2973-2973

[3] FrantsevaM V, CarlenP L, Perez VelazquezJ L. Dynamics of intracellular calcium and free radical production during ischemia in pyramidal neurons. Free Radic Biol Med, 2001, 31(10): 1216-1216

[4] NelsonJ D, wrightJ C. Conjunctival goblet cell densities in ocular surface disease. Arch Ophthalmol, 1984, 102(7): 1049-1049

[5] KunertK S, TisdaleA S, GipsonI K. Goblet cell numbers and epithelial proliferation in the conjunctiva of patients with dry eye syndrome treated with cyclosporine. Arch Ophthalmol, 2002, 120(3): 330-330

[6] KingL S, AgreP. Pathophysiology of the aquaporin water channels. Annu Rev Physiol, 1996, 58: 619-619

[7] ChoM L, KimW U, MinS Y, et al. . Cyclosporine differentially regulates interleukin-10, interleukin-15, and tumor necrosis factor a production by rheumatoid synoviocytes. Arthritis Rheum, 2002, 46(1): 42-42

[8] LiC, LimS W, SunB K, et al. . Expression of apoptosis-related factors in chronic cyclosporine nephrotoxicity after cyclosporine withdrawal. Acta Pharmacol Sin, 2004, 25(4): 401-401