A study on the traditional chinese medicine jinyebaidu for prevention and treatment of intrauterine infection with guinea pigs cytomegalovirus

Abstract

The purpose is to study the prophylactic and therapeutic effect of the traditional Chinese Medicine (TCM)-Jinyebaidu (JYBD) to guinea pig cytomegalovirus (GPCMV) intrauterine infection. The virus-free female and male guinea pigs were screened with nest-polymerase chain reaction (N-PCR). After inbred, pregnant guinea pigs were selected and divided into 3 groups randomly: 5 guinea pigs of the blank control group were not given either GPCMV or JYBD. 31 guniea pigs of the positive control group were inoculated 1 mL (10⁷ TCID₅₀) suspension of GPCMV intraperitoneal. 10 guniea pigs of the experimental group were inoculated GPCMV firstly and then perfused stomach with JYBD for 14 days, (Dosage in accordance with the modulus of the weight ratio of human to guniea pig). The effects of JYBD on the intrauterine infection of GPCMV were observed. The results showed that JYBD could decrease the maternal infection rate from 100% (31/31) to 50 % (5/10) (P<0.001), the intrauterine infection rate from 100% (72/72) to 75% (21/28) (P<0.001), and the rate of abnormal outcome of pregnancy from 64.4% (29/45) to 25.0% (7/28) (P<0.001), the infective symptoms being relieved. It can be concluded that traditional Chinese medicine-JYBD can prevent and treat GPCMV intrauterine infection, and can be expected a prophylactic drug for HCMV intrauterine infection.

Keywords

Jinyebaidu (JYBI)) / guinea pig cytomegalovirus (GPCMV) / intrauterine infection

References

[1] LeJ. Textbook for 21st Century: Obstetrics-Gynecology (Chinese), 20005th ed.Beijing, People's Medical Publishing House: 12-196

[2] ShiX Y. Modern Medical Laboratory Animal Science (Chinese), 2001, Beijing, People's Military Medical Press: 1-335

[3] BratcherD F, BourneN, BravoF J, et al. . Effect of Passive Antibody on Congenital Cytomegalovirus Infection in Guinea Pigs. J Infect Dis, 1995, 172: 944-944

[4] HarrisonC J, BrittW J, ChapmanN M, et al. . Reduced Congenital Cytomegalovirus Infection after Maternal Immunization with a Guinea Pig CMV Glycoprotein before Gestational Primary CMV Infection in the Guinea Pig Model. J Infect Dis, 1995, 172: 1212-1212

[5] GriffithB P, HsiungG D. Cytomegalovirus Infection in the Guinea Pigs, Maternal Infection at different stages of gestation. J Infect Dis, 1980, 141: 787-787

[6] KumarM L, NankervisG A. Experimental Congenital Infection with Cytomegalovirus: A Guinea Pig Model. J Infect Dis, 1978, 138: 650-650

[7] BiaF J, GriffithB P, FongC K Y, et al. . Cytomegalovirus infection in the Guinea Pig: Experimental models for Human Disease. Rev Infect Dis, 1983, 5: 177-177

[8] DaiD Y. Practical New & Special Drug Manual (Chinese), 19972nd ed.Beijing, People's Military Medical Press: 267-267

[9] XingW, WenL Z, DongJ H, et al. . Clinical an experimental study on antiviral activity of Reduqing against human cytomegalovirus. J Integr Tradit West Med (Chinese), 2000, 4(20): 215-215

[10] JiangH, WenL Z, LingX Z, et al. . The experimental study of inhibitory effects of Reduqing on human cytomegalovirus. J Exp Clin Virol (Chinese), 1999, 2(13): 175-175

[11] JiangH Z, LuY P, DongJ H, et al. . The experimental study on antiviral activity of Reduqing against respiratory syncytial virus and Influenza virus. Acta Tongji Med Univ (Chinese), 1996, 25: 28-28