A comparative study of^99mTc-YIGSR and^99mTc-MIBI uptake in tumor cells

Abstract

To investigate a new kind of tumor tracer^99mTc-YIGSR developed from a five amino structure (YIGSR) of the Laminin-chain, which can bind to the laminin receptors of tumor specifically, and radiolabeled with MAG₃. (1) Preparation of the^99mTc-YIGSR probe: with S-Acetly-NH₃-MAG₃ as the chelator and with proper reductants YIGSR was labeled with^99mTc; (2) Cell culture and viability measurement: EAC was maintained in RPMI 1640 supplemented with calf serum; the trypan blue exclusion was applied to calculate the cell viability; (3) Study of the cell dynamic: The EAC's uptake of^99mTc-YIGSR and^99mTc-MIBI was observed at 37 °C and 22 °C, respectively. (1) The labeling efficiencies of^99mTc-YIGSR and^99mTc-MIBI were (62±3)% and (96±2)%, respectively; (2) The cell viability was declined with time of incubation; (3) At 37 °C, the EAC'S uptake of^99mTc-YIGSR and^99mTc-MIBI reached the peak of (43.16±2.4)% and (24.4±1.8)% at 60 min, respectively; and at 22 °C, the highest uptake was (26.5±2.1)% and (9.47±1.9)% at 60 min, respectively. Thein vitro study suggests that^99mTc-YIGSR is superior to^99mTc-MIBI in cell uptake and has potential value in tumor imaging.

Keywords

laminin-YIGSR / methoxyisobuty (MIBI-lisonitrile) / tumor / cell uptake / ^99mTc

References

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