Role of β-adrenergic signal transduction pathway on myocardial ischemic preconditioning of rats

Abstract

To study the changes in every part of the β-adrenergic signal transduction pathway and their effects on ischemic preconditioning of rat myocardiumin vivo. SD rats were divided into three groups: IP group, I/R group and CON group. The IP group was further divided into PC1−, 2−, 3−, and PC1+, 2+, 3+ groups according to preconditioning procedure. The rats received surgical procedure and underwent left coronary artery occlusion and reperfusion. We analyzed the infarct size by TTC staining, measured serum myocardial enzymes, studied the β-AR Bmax and Kd by radioligand binding assay of receptors, checked the activity of AC and PKA by the method of biochemistry and examined the content of cAMP by radioimmunoassay. The infarct area was much smaller in the IP group than in the I/R group (P<0.001), while the enzymes were significantly higher in I/R (P<0.001). The Bmax of β-AR in IP was much higher than that in I/R (P<0.001), but no difference in Kd could be seen between IP and I/R groups. In IP, the activity of AC and PKA and the content of cAMP were higher than those in I/R (P<0.05, 0.002 and 0.001, respectively). In the procedure of preconditioning, the content of cAMP and the activity of PKA showed the characteristic of cyclic fluctuation. Ischemic preconditioning can protect the heart from necrosis and reduce endo-enzyme leakage. The system of β-adrenergic signal transduction pathway probably takes part in the protection effect of the IP, which might be clicited by the PKA.

Keywords

ischernic preconditioning / myocardial / receptors / adrenergic beta / cyclic AMP / cyclic AMP-dependent protein kinase / rat