Expression of heat shock protein 70 and 27 in non-small cell lung cancer and its clinical significance

Abstract

The heat shock proteins (HSPs) 70 and HSP 27 expression in patients with non-small cell lung cancer (NSCLC) was studied and the relationship between HSP 70 and HSP 27 with the clinicopathological features of NSCLC was investigated. The expression of HSP 70 and HSP 27 was detected in tumor tissues from 60 patients with NSCLC by S-P immunohistochemistry. The findings were analyzed in combination with the histological types, histopathological differentiation, lymph node metastasis, patients clinical stages, smoking history and gender. The results showed that of the 60 NSCLC tissue specimens studied, the immunoreactivity of HSP 70 and HSP 27 was detected in 47 (78.3%) and 43 (71.7%) specimens, respectively. A positive correlation was found between the overexpression of HSP 70 and HSP 27. The histopathological differentiation, lymph node metastasis, clinical stages and smoking history were correlated to the expression of HSP 70, but not to the expression of HSP 27. No statistical significance was observed in histological types and gender with respect to both HSP 70 and HSP 27 expression. It is suggested that the HSP 70 expression is a powerful and significant prognostic indicator and is related to histopathological differentiation, lymph node metastasis, patients' clinical stages, smoking history, whereas HSP 27 expression is not.

Keywords

non-small cell lung cancer / heat shock protein 70 and 27 / immunohistochemistry

References

[1] LazarisA C, TheodoropoulosGE, DavarisP S, et al. . Heat shock protein 70 and HLA-DR molecules tissue expression: prognostic implications in colorectal cancer. Dis Colon Rectum, 1995, 38: 739-739

[2] MichilsA, RedivoM, de BeylV Z. Increased expression of high but not low molecular weight heat shock proteins in respectable lung carcinoma. Lung Cancer, 2001, 33: 59-59

[3] CioccaD R, FuquaS A, Lock-LimS, et al. . Response of human breast cancer cells to heat shock and chemotherapeutic drugs. Cancer Res, 1992, 52: 3648-3648

[4] NanbuK, KonishiI, KomatsuT, et al. . Expression of heat shock proteins HSP70 and HSP90 in endometrial carcinomas: correlation with clinicopathology, sex steroid receptor status and p53 protein expression. Cancer, 1996, 77: 330-330

[5] GressT M, Muller-PillaschF, WeberC, et al. . Differential expression of heat shock proteins in pancreatic carcinoma. Cancer Res, 1994, 54: 547-547

[6] Vayssier-TaussatM, CamilliT, AronY, et al. . Effects of tobacco smoke and benzo[a]pyrene on human endothelial cell and monocyte stress responses. J Physiol Heart Circ Physiol, 2001, 280: 1293-1293

[7] OesterreichS, HilsenbeckS G, CioccaD R. The small heat shock protein HSP27 is not an independent prognostic marker in axiallary lymph node-negative breast cancer patients. Clin Cancer Res, 1996, 2: 1199-1199

[8] CornfordP A, DodsonA R, ParsonsK F. Heat shock protein expression independently predicts clinical outcome in prostate cancer. Cancer Res, 2000, 60: 7099-7099

[9] EhrenfriedJ A, HerronB E, TowsendC M. Heat shock proteins are differentially expressed in human gastrointestinal cancer. Surg Oncol, 1995, 4: 197-197

[10] LangdonS P, RabiaszG J, HirstG L, et al. . Expression of heat-shock protein HSP27 in human ovarian cancer. Clin Cancer Res, 1995, 1: 1603-1603

[11] HsuP L, HsuS M. Abundance of heat shock proteins (HSP89, HSP60, and HSP27) in malignant cells of Hodgkin's disease. Cancer Res, 1998, 58: 5507-5507

[12] KingK L, LiA F, ChauG Y. Prognostic significance of heat shock protein 27 expression in hepatocellular carcinoma and its relation to histologic grading and survival. Cancer, 2000, 88: 2464-2464

[13] SartoC, BinzP A, MocarelliP. Heat shock proteins in human cancer. Electrophoresis, 2000, 21: 1218-1218