The construction and investigation of PLGA artificial bone by biomimetic mineralization

Abstract

To modify the surface property of poly lactide-co-glycolide (PLGA) by biomimetic mineralization to construct a new kind of artificial bone. PLGA films and 3-diamensional (3-D) porous scaffolds hydrolyzed in alkaline solution were mineralized in SBF for 14 days. The morphology and composition of the mineral grown on PLGA were analyzed with SEM, FTIR and XRD. The porosity of the scaffolds was detected by using the liquid displacement method. The compressive strength of the scaffolds was detected by using a Shimadzu universal mechanic tester. An obvious mineral coating was detected on the surface of films and scaffolds. The main component of the mineral was carbonated hydroxyapatite (HA) similar to the major mineral component of bone tissues. The porosity of the un-mineralized and mineralized porous scaffolds was (84.86±8.52) % and (79.70±7.70) % respectively. The compressive strength was 0.784±0.156 N/mm² in un-mineralized 3-D porous PLGA and 0.858±0.145 N/mm² in mineralized 3-D porous PLGA. There were no significant differences between the mineralized and un-mineralized scaffolds (P>0.05) in porosity and biomechanics. Biomimetic mineralization is a suitable method to construct artificial bone.

Keywords

Poly lactide-co-glycolide / Mineralization / artificial bone / Biomechanics

References

[1] OhS H, KangS G, KimE S, et al. . Fabrication and characterization of hydrophilic poly (lactic-co-glycolic acid)/poly(vinyl alcohol) blend cell scaffolds by meltmolding particulate-leaching method. Biomaterials, 2003, 24: 4011-4011

[2] LiP. Biomimetic nano-apatite coating capable of promoting bone ingrowth. J Biomed Mater Res, 2003, 66A: 79-79

[3] BarrereF, van der ValkC M, MeijerG, et al. . Osteointegration of biomimetic apatite coating applied onto dense and porous metal implants in femurs of goats. J Biomed Mater Res, 2003, 67B: 655-655

[4] OhgushiH, CaplanA I. Stem cell technology and bioceramics: from cell to gene engineering. J Biomed Mater Res, 1999, 48(6): 913-913

[5] MurphyW L, MooneyD J. Biomspired growth of crystalline carbonate apatite on biodegradable polymer substrata. J Am Chem Soc, 2002, 124(9): 1910-1910

[6] OyaneA, KimH M, FuruyaT, et al. . Preparation and assessment of revised simulated body fluids. J Biomed Mater Res, 2003, 65A(2): 188-188

[7] ShiG X, WangS G, BeiJ Z. Preparation of porous cell scaffolds of poly (L-lactic acid) and poly (L-lactic-co-glycolic acid) and the measurement of their porosities. J Functional Polymers (Chinese), 2001, 1: 7-7

[8] MaoC B, LiH D, CuiF Z, et al. . Biomimetic Synthesis of Inorganic Materials. Progress in Chemistry, 1998, 10(3): 246-246

[9] HidekiAoki. Science and Medical Applications of Hydroxyapatite, 1991, Tokyo, Takayama Press System Center Co., Inc.: 165-177

[10] SchoofH, ApelJ, HeschelI, et al. . Control of pore structure and size in freeze-dried collagen sponges. J Biomed Mater Res, 2001, 58(4): 352-352

[11] KwonI K, ParkK D, ChoiS W, et al. . Fibroblast culture on surface-modified poly(glycolide-co-epsilon-caprolactone) scaffold for soft tissue regeneration. J Biomater Sci Polym Ed, 2001, 12(10): 1147-1147