Expression of CD151 in human atherosclerotic artery and its implication

Abstract

To investigate the expression of CD151 in human atherosclerosed artery and explore its clinical implications, Western blot and immunohistochemical techniques were used to determine the protein expression of CD151 in arterial tissues with atherosclerosis taken from 36 patients, including 26 cases who received bypass operation for peripheral artery atherosclerosis and 6 cases who died from coronary heart disease. The expression of CD151 in normal artery tissues from 15 healthy organ donators were also measured to serve as control. The results showed that expression of CD151 protein in atherosclerotic arteries was significantly higher than that in normal artery. In atherosclerotic arteries, CD151 expression was localized in vascular smooth muscle cells (VSMCs) in all tunica media and in partial subintima, while in normal artery, sparse expression was found in tunica media near adventitia. It is concluded that high CD151 protein expression in artery is associated with atherosclerosis and CD151 plays an important role in the atherosclerosis related to VSMC. The expression of CD151 in human atherosclerotic artery depends on the extent of atherosclerotic damage, its independent of risk factors.

Keywords

CD151 / atherosclerosis / artery / vascular smooth muscle

References

[1] SerruV, Le NaourF, BillardM, et al. . Selective tetraspan-integrin complexes (CD81/alpha4beta1, CD151/alpha3beta1, CD151/alpha6beta1) under conditions disrupting tetraspan interactions. Biochem J., 1999, 340: 103-103

[2] SincockP M, Graham MaryhoferG, AshmanL K. Localization of the transmembrane 4 superfamily (TM4SF) member PETA-3 (CD151) in normal human tissues: comparison with CD9, CD63, and α5 β1 integrin. J Histoch Cytoch, 1997, 45: 515-515

[3] HasegawaH, NomuraT, KishimotoK, et al. . SFA-1/PETA-3 (CD151), a member of the transmembrane 4 superfamily, associates preferentially with α5β1 integrin and regulates adhesion of human T cell leukemia virus type 1-infected T cells to fibronectin. J Immunol, 1998, 161: 3087-3087

[4] KohnoM, HasegawaH, MiyakeM, et al. . CD151 enhances cell motility and metastasis of cancer cells in the presence of focal adhesion kinase. Int J Cancer, 2002, 97: 336-336

[5] YauchR L, BerditchevskiF, HarlerM B, et al. . Highly stoichiometric, stable, and specific association of integrin α3β1 with CD151 provides a major link to phosphatidylinositol 4-kinase, and may regulate cell migration. Mol Biol Cell Online, 1998, 9: 2751-2751

[6] Yanez-MoM, AlfrancaA, CabanasC, et al. . Regulation of endothelial cell motility by complexes of tetraspan molecules CD81/TAPA-1 and CD151/PETA-3 with α3β1 integrin localized at endothelial lateral junctions. J Cell Biol, 1998, 141: 791-791

[7] SterkL T, GeuijenC W, BergJ V, et al. . Association of the tetraspanin CD151 with the laminin-binding integrins α3β1, α6β1, α6β4 and α7β1 in cells in culture andin vivo. J Cell Science, 2001, 115: 1161-1161

[8] MorlaA O, MogfordJ E. Control of smooth muscle cell proliferation and pheno2 type by integrin signaling through focal adhesion kinase. Biochem Biophys Res Commun, 2000, 272(1): 2982302-2982302

[9] HanksS K, PolteT R. Signaling through focal adhesion kinase. Bioessays, 1997, 19: 137-137

[10] ZhangX A, BontragerA L, HemberM E. Transmembrane-4 superfamily proteins associate with activated protein kinase C (PKC) and link PKC to specific beta (1) integrins. J Biol Chem, 2001, 276: 250005-250005