Impact of MAPK cascade pathway and P53 pathway upon liver transplant

Abstract

The change and the role of MAPK cascade pathway and P53 pathway after liver transplantation were explored. Thirty-four punctured donor liver specimens and 10 normal liver specimens were classified as group A (no rejection,n=10), group B (mild/moderate acute rejection,n =10), group C (serious acute rejection,n=8), group D (chronic rejection/fibrosis,n=6) and group E (control,n=10). By using immunohistochemistry, the expression levels of mitogen activated protein kinase (MAPK), Ras and P53 proteins, and byin situ hybridization, MAPK and ras mRNA expression levels were detected. The results showed that the expression levels of MAPK and Ras proteins were increased by turns in groups A, B and C, and decreased by turns in groups D and E. The protein expression of P53 was higher in the treated groups. The expression of Ras, HSP70 mRNA was identical as that of protein. It is suggested that the MAPK cascade pathway and P53 pathway can protect the hepatocytes by different mechanisms after liver transplantation. MAPKs cascade pathway repairs hepatocyte injury or accelerates hepatocytes into proliferation or differentiation. P53 pathway blocks cell cycle within G1 phase to make hepatocyte repair or apoptosis to reduce disorder differentiation.

Keywords

liver transplantation / MAPK cascade pathway / P53

References

[1] IesalnieksI, RentschM, LengyelE, et al. . JNK and p38MAPK are activated during graft reperfusion and not during cold storage in rat liver transplantation. Transplant Proc, 2001, 33: 931-931

[2] LiuJ S. Cellular information and Modulation, 1998, Beijing, Combined Publishing House of Beijing Medical University and Chinese Union Medical University: 271-281

[3] YoshinariD, TakeyoshiI, KobayashiM, et al. . Effects of a p38 mitogen-activated protein kinase inhibitor as an additive to university of Wisconsin solution on reperfusion injury in liver transplantation. Transplantation, 2001, 72(1): 22-22

[4] XiaZ G, DickensM, RaingeaudJ, et al. . Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science, 1995, 279(5240): 1326-1326

[5] TatekawaY, KanehiroH, KanokogiH, et al. . Intragraft expression of p38 in rat small bowel transplantation. Transplant Proc, 2000, 32: 1281-1281

[6] RosenauJ, BahrM J, von WasielewskiR, et al. . Ki67, E-cadherin, and p53 as prognostic indicators of long-term outcome after liver transplantation for metastatic neuroendocrine tumors. Transplantation, 2002, 73(3): 386-386

[7] SheikhM S, ChenY Q, SmithM L, et al. . Role of p21WAF1/CIP1/Sdil in cell death and DNA repair as studied using a tetracycline-inducible system in p53-deficient cells. Oncogene, 1997, 14: 1875-1875