Expression of RECK gene and MMP-9 in hilar cholangiocarcinoma and its clinical significance

Abstract

In order to study the expression of transformation suppressor gene RECK and MMP-9 in hilar cholangiocarcinomas and its clinical significance, and explore the roles of RECK gene in metastasis and invasion of hilar cholangiocarcinoma, the expression levels of RECK, and MMP-9 mRNA were detected by using reverse transcription-polymerase reaction in 42 paraffin-embedded samples of hilar cholangiocarcinoma and 10 samples of benign bile duct diseases. The results showed that in hilar cholangiocarcinoma tissues, the expression of RECK gene was 0.235±0.062, significantly lower than in normal bile duct tissue (0.533±0.024,P<0.05). In hilar cholangiocarcinoma tissues, the expression of MMP-9 (0.528±0.039) was significantly higher than in the normal tissues (0.311±0.032,P<0.05). The expression of RECK gene was closely related to the intrahepatic and surrounding organs invasion (P<0.05). It was concluded that RECK gene could inhibit the expression of MMP-9 in hilar cholangiocarcinomas and closely correlated with the biological behaviors. The abnormal expression of RECK gene might be one of the molecular mechanisms of hilar cholangiocarcinoma metastasis.

Keywords

hilar cholangiocarcinoma / RECK / MMP-9

References

[1] BissellM J, RadiskyD. Putting tumors in context. Nat Rev Cancer, 2001, 1: 46-46

[2] XiaoM, ZhouN X, HuangZ Q, et al. . The ratio of MMP-2 to TIMP-2 in hilar cholangiocarcinoma: a semiquantitative study. Hepatobility Pancreat Dis Int, 2004, 3(4): 599-599

[3] KimH J, KimJ S, KangC D, et al. . Expression of epidermal growth factor receptor, ErbB2 and matrix metalloproteinase-9 in hepatolithiasis and cholangiocarcinoma. Korean J Gastroenterol, 2005, 45(1): 52-52

[4] TakahashiC, ShengZ, HoranT P, et al. . Regulation of matrix metalloproteinase-9 and inhibition of tumor invasion by the membrane-anchored glycoprotein RECK. Proc Natl Acad Sci U S A, 1998, 95(22): 13221-13221

[5] SasaharaR M, TakahashiC, NodaM, et al. . Involvement of the Sp1 site in ras-mediated downregulation of the RECK metastasis suppressor gene. Biochem Biophys Res Commun, 1999, 264(3): 668-668

[6] OhJ, TakahashiR, KondoS, et al. . The membrane-anchored MMP inhibitor RECK is a key regulator of extracellular matrix integrity and angiogenesis. Cell, 2001, 107(6): 789-789

[7] MasuiT, DoiR, KoshibaT, et al. . RECK expression in pancreatic cancer: its correlation with lower invasiveness and better prognosis. Clin Cancer Res, 2003, 9(5): 1779-1779

[8] SpanP N, SweepC G, MandersP, et al. . Matrix metallo-proteinase inhibitor reversion-inducing cysteine-rich protein with Kazal motifs: a prognostic marker for good clinical outcome in human breast carcinoma. Cancer, 2003, 97(11): 2710-2710