Hypoglycemic activity of Jatrorrhizine

Abstract

The hypoglycemic activity and its mechanism of Jatrorrhizine (Jat) were studied. The normal mice and alloxan-induced hyperglycemic mice were given with different doses of Jat. Blood glucose and liver glycogen levels were determined by spectrophotometry with glucose-oxidase and iodine reagents respectively. The levels of blood lactic acid (LC) and liver lactate dehydrogenase (LDH) activity were measured to explore the effect of Jat on anaerobic glycolysis. Succinate dehydrogenase (SDH) activity in liver was measured to evaluate the effect of Jat on aerobic glycolysis in liver. It was found that Jat (50 mg/kg, 100 mg/kg) could significantly decrease blood glucose level in a dose-and time-dependent manner in both normal and alloxan-diabetic mice, increase the activity of SDH, but had no significant effects on the LC level and LDH activity. Jat could significantly reduce the content of liver glycogen in normal mice. Moreover, Jat could inhibit the platelet aggregation in rabbitsin vitro in a dose-effect relationship. It was concluded that Jat induced the pronounced decrease in blood glucose in normal and hyperglycemic mice. The hypoglycemic activity of Jat may be attributed to the enhancement of aerobic glycolysis.

Keywords

jatrorrhizine / hypoglycemia / blood glucose / succinate dehydrogenase / liver glycogen / platelet aggregation

References

[1] ChenQ M, XieM Z. Studies on the hypoglycemic effect ofCoptis Chinesis and berberine. Acta Pharmaceutica Sinica (Chinese), 1986, 21(6): 401-401

[2] NiY X, LiuA Q, GaoY F, et al. . Therapeutic effet of Berberine on 60 patients with type II diabetes mellitus and experimental researh. Chin J Integr Med (Chinese), 1988, 8(12): 711-711

[3] FengR F. Serum glucose determination by glucose-oxidase and peroxidase. Shanghai Med (Chinese), 1980, 12: 43-43

[4] XuS Y. Experimental Methods of Pharmacology (Chinese), 1982, Beijing, People's Sanitation Press: 960-962

[5] FriedemannT E, HaugenG E. Pyruvic acid determination of keto-acetic acids in blood and urine. J Biol Chem, 1943, 147: 415-415

[6] Phylip GcabaudH, WroblewskiF. Colorimetric measurement of lactic dehydrogenase activity of body fluids. Am J Clin Pathol, 1953, 30: 234-234

[7] DaiG H, YangT S. Study on respiration of cardiac muscle. Clin J Endomiol (Chinese), 1985, 4: 78-78

[8] XuS Y. Experimental Methods of Pharmacology (Chinese), 1982, Beijing, People's Sanitation Press: 1518-1519

[9] ChenQ. Pharmacological Methodology of Chinese Medicine, 1996, Bejing, People's Sanitation Press: 131-132

[10] HanH, FangD C. The blocking and partial agonistic actions of jatrorrhizine on alpha-adrenoceptors. Acta Pharmacologica Sinica, 1989, 10(5): 385-385