Screening of anti-endotoxin components fromRadix isatidis

Abstract

To find a component with the strongest anti-endotoxin effect from the four components (F₀₂₁, F₀₂₂, F₀₂₃, and F₀₂₄) of chloroform extract (F₀₂) ofRadix Isatidis, the protective effects of the five components on endotoxin-challenged mice and their effects on the production of TNF_α and IL-6 by macrophages from the endotoxin-challenged mice were compared. It was found that the five components all had protective effects on endotoxin-challenged mice in terms of mortality. The mortalities of the mice challenged by 2.42 mg/kg endotoxin were 30%, 50%, 20%, 50% and 60% after treatment with F₀₂, F₀₂₂, F₀₂₃, and F₀₂₄. The 5 components all had inhibotory effects on the production of TNF_α and IL-6 by macrophages from the mice. At a concentration of LPS of 50 ng/mL, the inhibitory rates of F₀₂, F₀₂₁1, F₀₂₂, F₀₂₃, and F₀₂₄ for the production of TNF_α and IL-6 were 76.54%, 30.86%, 78.60%, 36.63%, 2.06% and 75.85%, 18.45%, 77.68%, 24.41%, 3.64% and at the dose of 100 ng/mL, the inhibitory rates were 49.65%, 16.86%, 66.97%, 13.39%, 7.16% and 59.78%, 1.28%, 61.86%, 2.08%, 1.44%, respectively. It is concluded that the component F₀₂₂ ofRadix Isatidis has the strongest anti-endotoxin effect.

Keywords

Radix Isatidis / endotoxin / activity / mortality / TNF_α / IL-6 / mice