Effects of amiodarone on transmural dispersion of ventricular effective refractory periods across myocardial layers in the normal and hypertrophic canine heart

Abstract

The effects of amiodarone on transmural dispersion of ventricular effective refractory periods (ERPs) in the normal and hypertrophic canine heart were investigatedin vivo. By using the programmed stimulation protocol, the ERPs of epicardium (Epi), midmyocardium (Mid) and endocardium (Endo) were measured by inserting specially-designed electrodes into the three myocardial layers before and after mainlining of amiodarone. No significant ERPs-dispersion was observed in the three layers before and after mainlining of amiodarone in the normal group. In contrast, ERPs of all the three layers were prolonged in the hypertrophic heart, while the ERPs-dispersion was reduced significantly after mainlining of amiodarone. The ERPs-dispersion was significantly increased in the hypertrophic heart but not in the normal heart using “long-short” and “short-long” interval stimulation technique. It was concluded that (1) the differences in ERPs-dispersion among the three layers were significant in hypertrophic heart, and differences were not significant in normal canine heart; (2) ERPs of each three-myocardial layers were significantly prolonged after using amiodarone, but the ERPs-dispersion decreased in hypertrophic heart and (3) the programmed extrastimulus technique of “long-short” and “short-long” intervals increased the transmural ERPs-dispersion in the hypertrophic heart.

Keywords

amiodarone / myocardium / hypertrophy / transmural dispersion of ventricular effective refractory periods

References

[1] BrockmanS K. Cardiodynamics of complete heart block. Am J Cardiol, 1965, 16: 72-83

[2] BalR, LüJ G, PuJ, et al. . Influence of pacing site on myocardial transmural dispersion of repolarization in normal and long QT dog. Chin J Cardiac Arrhyth (Chinese), 2004, 10: 278-284

[3] ScicouriS, AntzelevitchC. Electrophysiologic characteristics of M cells in the canine left ventricular free wall. J Cardiovasc Electrophysiol, 1995, 6: 591-603

[4] SicouriS, FishJ. Antzelevitch C. Distribution of M cells in the canine ventricle. J Cardiovasc Electrophsiol, 1994, 5: 824-837

[5] LüJ G, LuZ Y, FrederikVoss, et al. . Experimental Study of ERP-Dispersion among myocardial layers in the intact normal and hypertrophic canine heart. Chin J Cardiol (Chinese), 2001, 10: 609-611

[6] MerotJ, CharpentierF, PoirierJ M, et al. . Effects of chronic treatment byamiodarone on transmural heterogeneity of canine ventricular repolarization in vivo: interactions with acute sotalol. Cardiovasc Res, 1999, 44: 303-314