Study on the protective effects of compound blood-activating soup on bone marrow hematopoietic cells in acute radiation injured mice

Abstract

After irradiation with 8 Gy⁶⁰Co γ-ray, mice were immediately given intraperitoneal injection of 200 mg 100% compound blood-activating soup twice a day. On the 3rd and 7th day, the P₅₃ gene expression of bone marrow hematopoietic cells in Chinese drug group was found to be higher than that in normal group, and it was also significantly higher than that in control group. The expression level of GADD₁₅₃ gene which was not expressed in normal group was much lower in Chinese drug group than that in control group. On the 7th day after irradiation, the P53 and GADD153 gene expression levels of splenic mononuclear cells were consistent with those of bone marrow hematopoietic cells both in Chinese drug group and control group. On the 3rd and 7th day, the bone marrow hematopoietic tissue volume in Chinese drug group was higher than that in control group, with no difference found between the two groups. While on the 14th day, the difference became significant (P<0.01). The results showed that commonly used blood-activating and stasis-eliminating drugs may strengthen the viability of hematopoietic cells and promote the rehabilitation of hematopoiesis by inducing wt-P₅₃ expression to block the bone marrow hematopoietic cells in G₁ phase, during which DNA could be repaired.

Keywords

acute radiation injury / hematopoietic cells P₅₃ gene / GADD₁₅₃ gene / blood-activating and stasis-eliminating Chinese drugs

References

[1] 1: 1981,2(2):1111

[2] 1997,17(5):50

[3] 1993.59

[4] ChomczynskiP, SacchiN. Single-step Method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem, 1987, 162: 156-156

[5] 1996,41:58

[6] 60Coγ 1996,4:195

[7] EyminB, DubreL, SolaryE, et al. . Relationship between GADD₁₅₃ gene expression and DNA fragmentation in leukemic cells, sensitive to apoptosis induced by VP16. Blood, 1995, 86: 758-758