Expression of adherent molecule and cyclin by ligustrazine in bone marrow of mice with immune-induced aplastic anemia

Abstract

Mice with immune-induced aplastic anemia (AA) were given 5 mg ligustrazine intraperitoneally twice a day. On the 14th day, the expression of CD_49d CD_49c cyclinD₂ in bone marrow mononuclear cells (MNC) was examined by flow cytometry, and VCAM-1 on stromal cells was immunohistochemically measured by Strept Avidin-Biotin Complex (SABC). The expression of CD_49d, CD_49c, VCAM-1 and cyclinD₂ in ligustrazine-treated group was significantly higher than that in AA group (P< 0. 01), but the ratio of G₀+G₁ phase cells was significantly lower than that in A A group (P< 0. 01). The results showed that ligustrazine could improve the expression of adherent molecule and cyclin D₂ in the bone marrow of mice with immune-induced aplastic anemia, thereby promoting the growth of hematopoietic cells.

Keywords

anemia / anemia aplastic / adherent molecules / ligustrazine / cyclin

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