Effects of nerve growth factor on axonal retrograde transport after axonal injury of motoneurons

Abstract

In this experimental study we coupled nerve growth factor (NGF) with notatin by dicyclohexylcarbodiimide to label NGF and observed the effects of NGF on axonal retrograde transport after axonal injury of motoneurons with the aid of notatin chemiluminescence system. The results showed that NGF could be transported through axon not only to sensory neurons in dorsal root ganglia but also to motoneurons in spinal cord ventral horn.

Keywords

nerve growth factor / retrograde transport / chemiluminescence

References

[1] StöckelK, SchwabM E, ThoenenH. Specificity of retrograde transport of nerve growth factor (NGF) in sensory neurons: a biological and morphological study. Brain Res, 1975, 89: 1-1

[2] FitzgeraldM, WallP D, GoedertM, et al. . Nerve growth factor counteracts the neurophysiological and neurochemical effects of chronic sciatic nerve section. Brain Res, 1985, 332: 131-131

[3] YanQ, JohnenE M. An immunonistochemical study of the nerve growth factor receptor in developing rats. J Neurosci, 1988, 8: 3481-3481

[4] ErnforsP, HenschenA, OlsonL, et al. . Expression of nerve growth factor receptor mRNA is developmentally regulated and incresed after axotomy in rat spinal cord motoneuroms. Neuron, 1989, 2: 1605-1605

[5] RendeM, HaggT, ManthorpeM, et al. . Nerve growth factor receptor immunoreactivity in neurons of the normal adult rat spinal cord and its modulation affter peripheral nerve lesions. J Comp Neurol, 1992, 319: 285-285

[6] MillerM S, BuckS H, SipesT, et al. . Regulation of substance P by nerve growth factor disruption by capsaicin. Brain Res, 1982, 250: 193-193

[7] SaikaT, SenbE, NoguchiK, et al. . Effects of nerve crush and transection on mRNA levesls for nerve growth factor receptor in teh rat facial motoneurons. Brain Res Mol Brain Res, 1991, 9: 157-157

[8] JohnsonE M, RichE M, YipH K, et al. . The rolo of NGF in sensory neuronsin vivo. Trends Neurosci, 1986, 9: 33-33

[9] BothwellM A, SchechterA L, VanghnK M, et al. . Clonal variants of PC₁₂ Pheochromocytoma cells with altered response to nerve growth factor. Cell, 1980, 21: 857-857

[10] BradshawR A. Nerve growth factor. Annu Rev Biochem, 1978, 47: 191-191