Construction of eukaryotic expression vector pBlacZ and its expression bothIn Vitro andIn Vivo

Abstract

A novel eukaryotic expression vector pBlacZ was constructed, which was transfected into the cell lines of NIH/3T3, COS-1, CHO and the primary culture of murine dermatic fibroblastsin vitro, and also into the murine subcutaneous layer and skeletal muscles of ratsin vivo. It was detected that the gene expression vector could encode theE. Coli β-galactosidase effectively in all these histocytes. The results suggested that pBlacZ, as a novel expression vector, might have certain value of application.

Keywords

DNA recombination / gene transfers gene expression / β-galactosidase