The inhibitory effects of IFN α-2b on EGFR expression and growth of cultured subconjunctival fibroblasts

Abstract

To investigate to what extent the growth of cultured subconjunctival fibroblasts was regulated by epidermal growth factor receptor (EGFR). we observed the expression of EGFR inhibited by interferon alpha-2b (IFN α-2b) and determined the growth of cultured subconjunctival fibroblasts by immunohistochemical and MTT methods. The linear relationships between the concentration of IFN α-2b and EGFR expression or growth were y=1252. 7- 554. 61 lgx (r = - 0. 98,P <0.01), y = 0.523−0.0421 lgx (r=−0.93,P <0.05) respectively. The concentration of IFN α-2b at doubled IC₅₀ to EGFR (6. 55 × 10³ IU/ml) only inhibited the cell growth by 25. 13%. The results suggested that the growth of subconjunctival fibroblasts may be controlled by many other growth factors because it was only partially inhibited by decreasing EGFR expression.

Keywords

fibroblast / cultured / EGFR / interferon alpha-2b

References

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