Effects of pHGF on hepatocyte DNA synthesis after partial hepatectomy in rats
Tang Wangxian , Liang Kuohuan , Wang Junping , Du Lijing , Zhang Wenying
Current Medical Science ›› 1998, Vol. 18 ›› Issue (1) : 25 -27.
The effects of pHGF on the changes of hepatocyte proliferative cycle and liver regenerative capacity after partial hepatectomized rats were observed by flow cytometry (FCM). The results were as follows: 1) S phase fraction (SPF) in group of normal rats (group A) accounted for 9. 89 % and increased gradually within 6 h, following a peak at 12th h or 36th h after operation, but in the group of pHGF-treated rats (group B) the peak appeared at 24th h after operation; 2) Proliferation index (PI) of group A was 19. 6 % before partial hepatectomy, increased to 34. 91 % within 6 h and reached a peaks at 12th or 36th h after operation, and in group B the peak appeared at 48th h after operation. There were significant differences between two groups in SPF and PI (P<0. 01). The weight of liver began to increase 12 h after operation, and almost reached the preoperative weight 5 days after operation. These findings suggest that pHGF can promote the DNA synthesis and segmentation of hepatocyte.
partial hepatectomy / DNA / flow cytometry
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