Effects of cyclosporin a on proliferation of cultured rat mesangial cells

Abstract

The proliferation of mesangial cells on cyclosporin (CsA) test medium was studied by MTT assay and TNF-α in cultured supernatant was examined by using ELISA. The results showed that cyclosporin A significantly inhibited the proliferation of mesangial cells at the concentration between 0. 25 – 15 μg/ml (IC50 1 μg/ml). This action appeared to be dose-dependent. Release of TNF-α from mesangial cells stimulated by LPS was also dose-dependently suppressed. It is suggested that cyclosporin A play an important role in antiprohferation mechanism of mesangial cellsin vitro.

Keywords

cyclosporin A / mesangial cells / tumor necrosis factor-α

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